Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), Institute of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China.
National & Local Joint Engineering Research Center for the Exploition of Homology Resources of Southwest Medicine and Food, Guizhou University, Guiyang, China.
Biomed Res Int. 2021 Apr 29;2021:5562461. doi: 10.1155/2021/5562461. eCollection 2021.
is a medicinal, ornamental, and edible plant, and its essential oil has been used as an aromatic stomachic in China. In this study, the chemical constituents, antibacterial, and anti-inflammatory properties of flower essential oil (F-EO), leaf essential oil (L-EO), and stem essential oil (S-EO) of were investigated for the first time. According to the GC-FID/MS assay, the F-EO was mainly composed of bornyl formate (21.7%), 1,8-cineole (21.6%), borneol (9.7%), methyleugenol (7.7%), -myrcene (5.4%), limonene (4.7%), camphene (4.5%), linalool (3.4%), and -pinene (3.1%). The predominant components of L-EO were bornyl formate (33.9%), borneol (13.2%), 1,8-cineole (12.1%), methyleugenol (8.0%), camphene (7.8%), bornyl acetate (6.2%), and -pinene (4.3%). The main components of S-EO were borneol (22.5%), 1,8-cineole (21.3%), methyleugenol (14.6%), bornyl formate (11.6%), and bornyl acetate (3.9%). For the bioactivities, the F-EO, L-EO, and S-EO exhibited significant antibacterial property against , , , , , and with the inhibition zones (7.28-9.69 mm), MIC (3.13-12.50 mg/mL), and MBC (6.25-12.50 mg/mL). Besides, the F-EO, L-EO, and S-EO significantly inhibited the production of proinflammatory mediator nitric oxide (NO) (93.15-94.72%) and cytokines interleukin-6 (IL-6) (23.99-77.81%) and tumor necrosis factor- (TNF-) (17.69-24.93%) in LPS-stimulated RAW264.7 cells at the dose of 128 g/mL in the absence of cytotoxicity. Hence, the essential oils of flower, leaf, and stem could be used as natural antibacterial and anti-inflammatory agents with a high application potential in the pharmaceutical and cosmetic fields.
是一种药用、观赏和食用植物，其精油在中国一直被用作芳香健胃剂。本研究首次对 的花精油（F-EO）、叶精油（L-EO）和茎精油（S-EO）的化学成分、抗菌和抗炎特性进行了研究。根据 GC-FID/MS 分析，F-EO 主要由莰烯甲酸酯（21.7%）、1,8-桉叶素（21.6%）、龙脑（9.7%）、甲基丁香酚（7.7%）、-月桂烯（5.4%）、柠檬烯（4.7%）、莰烯（4.5%）、芳樟醇（3.4%）和 -蒎烯（3.1%）组成。L-EO 的主要成分是莰烯甲酸酯（33.9%）、龙脑（13.2%）、1,8-桉叶素（12.1%）、甲基丁香酚（8.0%）、莰烯（7.8%）、乙酸龙脑酯（6.2%）和 -蒎烯（4.3%）。S-EO 的主要成分是龙脑（22.5%）、1,8-桉叶素（21.3%）、甲基丁香酚（14.6%）、莰烯甲酸酯（11.6%）和乙酸龙脑酯（3.9%）。在生物活性方面，F-EO、L-EO 和 S-EO 对 、 、 、 、 和 具有显著的抗菌活性，抑菌圈（7.28-9.69mm）、最小抑菌浓度（MIC）（3.13-12.50mg/mL）和最小杀菌浓度（MBC）（6.25-12.50mg/mL）。此外，F-EO、L-EO 和 S-EO 可显著抑制 LPS 刺激 RAW264.7 细胞产生的促炎介质一氧化氮（NO）（93.15-94.72%）和细胞因子白细胞介素-6（IL-6）（23.99-77.81%）和肿瘤坏死因子-（TNF-）（17.69-24.93%），剂量为 128μg/mL 时无细胞毒性。因此， 的花、叶和茎精油可作为天然抗菌和抗炎剂，在制药和化妆品领域具有很高的应用潜力。
