National & Local Joint Engineering Research Center for the Exploition of Homology Resources of Southwest Medicine and Food, Guizhou University, Guiyang 550025, China.
College of Life Sciences, Guizhou University, Guiyang 550025, China.
Molecules. 2020 Dec 31;26(1):167. doi: 10.3390/molecules26010167.
W. W. Smith, an edible, medicinal, and ornamental plant, is mainly cultivated in China and Myanmar. The essential oil (EO) from rhizome has been used as an aromatic stomachic in China. The chemical composition and biological activities of EO from rhizome were reported for the first time. Based on gas chromatography with flame ionization or mass selective detection (GC-FID/MS) results, the major constituents of EO were 1,8-cineole (47.6%), borneol (15.0%), methyleugenol (11.2%), and bornyl formate (7.6%). For bioactivities, EO showed a significant antibacterial activity against , , , , , and with the diameter of the inhibition zone (DIZ) (8.66-10.56 mm), minimal inhibitory concentration (MIC) (3.13-6.25 mg/mL), and minimal bactericidal concentration (MBC) (6.25-12.5 mg/mL). Moreover, EO (128 μg/mL) significantly inhibited the production of proinflammatory mediators nitric oxide (NO) (92.73 ± 1.50%) and cytokines tumor necrosis factor-α (TNF-α) (20.29 ± 0.17%) and interleukin-6 (IL-6) (61.08 ± 0.13%) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages without any cytotoxic effect. Moreover, EO exhibited significant acetylcholinesterase (AChE) inhibitory activity (the concentration of the sample that affords a 50% inhibition in the assay (IC) = 1.03 ± 0.18 mg/mL) and moderate α-glucosidase inhibition effect (IC = 11.60 ± 0.25 mg/mL). Thus, the EO could be regarded as a bioactive natural product and has a high exploitation potential in the cosmetics and pharmaceutical industries.
食用、药用和观赏植物 W. W. Smith 主要在中国和缅甸种植。根茎的精油在中国被用作芳香性健胃药。首次报道了根茎精油的化学成分和生物活性。基于气相色谱法与火焰离子化或质量选择检测（GC-FID/MS）结果，精油的主要成分是 1,8-桉叶油醇（47.6%）、龙脑（15.0%）、甲基丁香酚（11.2%）和硼酸酯（7.6%）。在生物活性方面，精油对 、 、 、 、 表现出显著的抗菌活性，抑菌圈直径（DIZ）（8.66-10.56mm）、最小抑菌浓度（MIC）（3.13-6.25mg/mL）和最小杀菌浓度（MBC）（6.25-12.5mg/mL）。此外，精油（128μg/mL）显著抑制脂多糖（LPS）诱导的 RAW264.7 巨噬细胞中促炎介质一氧化氮（NO）（92.73±1.50%）和细胞因子肿瘤坏死因子-α（TNF-α）（20.29±0.17%）和白细胞介素-6（IL-6）（61.08±0.13%）的产生，而没有任何细胞毒性作用。此外，精油表现出显著的乙酰胆碱酯酶（AChE）抑制活性（在测定中产生 50%抑制的样品浓度（IC）=1.03±0.18mg/mL）和适度的α-葡萄糖苷酶抑制作用（IC=11.60±0.25mg/mL）。因此，精油可以被视为一种具有生物活性的天然产物，在化妆品和制药行业具有很高的开发潜力。
