Department of Medical Physiology, College of Medicine, Zagazig University, Al-Sharquia 44519, Egypt.
Department of Physiology, College of Medicine, Qassim University, Buraidah 52645, Saudi Arabia.
Biomed Res Int. 2021 Apr 26;2021:5570229. doi: 10.1155/2021/5570229. eCollection 2021.
The reduction in estrogen levels results in a decrease in bone density at menopause. Irisin is a myokine that modulates the benefits of exercise, which may include bone health. This study was planned to examine irisin's impact in preventing osteoporosis after ovariectomy. 4 groups of female albino rats (10 rats/group): control, sham-operated, ovariectomized (OVX-control), and OVX-irisin-treated. Serum levels of bone markers [osteocalcin (OC), bone alkaline phosphatase (BALP), tartrate-resistant acid phosphatase (TRAP), calcium (Ca), phosphorus (P)], glucose, and insulin were being measured. Body mass index, Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), dry and ash femur weight, and bone contents of Ca and P were investigated. The femur was examined histopathologically. The OVX-control group showed an increase in serum levels of OC, BALP, TRAP, calcium, phosphorus, BMI, glucose, insulin, and HOMA-IR ( < 0.05) and a reduction in dry and ash weight of the femur, the concentration of calcium and phosphorus content in bone ash ( < 0.05). The OVX-irisin-treated group exhibited a decrease in serum levels of OC, BALP and TRAP, calcium, phosphorus, BMI, glucose, insulin, HOMA-IR ( < 0.05), and a rise in dry and ash weight of the femur, the concentration of calcium and phosphorus in bone ash ( < 0.05). Histological examination of the distal femur diaphysis of the OVX-irisin-treated group exhibited proper bone architecture and density compared with that of the OVX-control group. It is concluded that irisin treatment in the OVX rats safeguarded the regular bone architecture and normal levels of serum bone biomarkers. Irisin may be a possible novel target in the prohibition of postmenopausal osteoporosis.
雌激素水平降低会导致绝经期骨质密度下降。鸢尾素是一种肌因子，可调节运动的益处，包括骨骼健康。本研究旨在探讨鸢尾素在预防卵巢切除术后骨质疏松症中的作用。将 4 组雌性白化大鼠（每组 10 只）分为 4 组：对照组、假手术组、卵巢切除组（OVX-对照组）和卵巢切除+鸢尾素处理组。测量血清骨标志物[骨钙素（OC）、骨碱性磷酸酶（BALP）、抗酒石酸酸性磷酸酶 5b（TRAP）、钙（Ca）、磷（P）]、血糖和胰岛素水平。测量体重指数（BMI）、胰岛素抵抗指数（HOMA-IR）、股骨干重和灰重以及股骨 Ca 和 P 含量。对股骨进行组织病理学检查。OVX-对照组血清 OC、BALP、TRAP、Ca、P、BMI、血糖、胰岛素和 HOMA-IR 水平升高（<0.05），股骨干重和灰重、骨灰中 Ca 和 P 含量降低（<0.05）。OVX-鸢尾素处理组血清 OC、BALP 和 TRAP、Ca、P、BMI、血糖、胰岛素和 HOMA-IR 水平降低（<0.05），股骨干重和灰重、骨灰中 Ca 和 P 含量升高（<0.05）。与 OVX-对照组相比，OVX-鸢尾素处理组大鼠股骨远端干骺端的组织学检查显示骨结构和密度正常。结论：OVX 大鼠的鸢尾素治疗可保护正常的骨结构和血清骨标志物水平。鸢尾素可能是预防绝经后骨质疏松症的一个新的潜在靶点。
