Department of General Medicine, Foshan First People's Hospital, Foshan City, Guangdong Province, 528000, China.
Department of Infectious Disease, Foshan First People's Hospital, Foshan City, Guangdong Province, 528000, China.
Biomed Res Int. 2021 Apr 20;2021:5573469. doi: 10.1155/2021/5573469. eCollection 2021.
To investigate the expression and clinical significance of miR-146a and tumor necrosis factor receptor-associated factor 6 (TRAF6) in myasthenia gravis (MG) patient serum.
The serum of 52 patients with MG and 60 healthy individuals was collected in our hospital. The expression of miR-146a and TRAF6 in serum was measured by real-time PCR (RT-PCR). Comparison among serum miR-146a and TRAF6 mRNA group clinical characteristics and assorted expressions was done with the correlation among the two groups evaluated. Logistics regression was used to analyze the effect of miR-146a and TRAF6 mRNA on MG development with the ROC curve applied for an investigation into the diagnostic role of miR-146a and TRAF6 mRNA expression in MG development.
miR-146a and TRAF6 mRNA were significantly increased in the patients with MG compared with the healthy controls. Significant differences were identified in respiratory muscle endurance, muscle weakness level, vital capacity, and maximal voluntary ventilation between the two groups. Additionally, correlation analysis has discovered a positive correlation between miR-146a and TRAF mRNA expression in patients with MG. miR-146a and TRAF6 mRNA are independent MG occurrence factors exhibited by multivariate analysis while areas under ROC curve (AUCs) of miR-146a and TRAF6 mRNA in MG diagnosis were established by ROC curve analysis with results being 0.782 and 0.703, correspondingly.
miR-146a and TRAF6 mRNA are highly expressed in MG patients and can affect MG occurrence. miR-146a is a suitable candidate marker for diagnosing MG.
研究 miR-146a 和肿瘤坏死因子受体相关因子 6（TRAF6）在重症肌无力（MG）患者血清中的表达及其临床意义。
收集我院 52 例 MG 患者和 60 例健康个体的血清。采用实时 PCR（RT-PCR）检测血清中 miR-146a 和 TRAF6 的表达。比较两组血清 miR-146a 和 TRAF6mRNA 组的临床特征和表达情况，评估两组之间的相关性。采用逻辑回归分析 miR-146a 和 TRAF6mRNA 对 MG 发病的影响，ROC 曲线分析 miR-146a 和 TRAF6mRNA 表达在 MG 发病中的诊断作用。
与健康对照组相比，MG 患者的 miR-146a 和 TRAF6mRNA 显著升高。两组间呼吸肌耐力、肌无力程度、肺活量和最大自主通气量存在显著差异。此外，相关性分析发现 MG 患者 miR-146a 和 TRAFmRNA 表达呈正相关。多因素分析显示，miR-146a 和 TRAF6mRNA 是独立的 MG 发病因素，ROC 曲线分析结果显示 miR-146a 和 TRAF6mRNA 在 MG 诊断中的 AUC 分别为 0.782 和 0.703。
miR-146a 和 TRAF6mRNA 在 MG 患者中高表达，可影响 MG 的发生。miR-146a 是诊断 MG 的一个合适的候选标志物。
