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变种叶绿体基因组的特征分析与系统发育分析

Characterization and phylogenetic analysis of the chloroplast genome of var. .

作者信息

Cai Xiaoran, Zhang Bin, Wang Shuai, Cheng Yueqin, Wang Hongwei

机构信息

College of Plant Protection, Henan Agricultural University, Zhengzhou, China.

College of Life Science, Henan Agricultural University, Zhengzhou, China.

出版信息

Mitochondrial DNA B Resour. 2021 Apr 27;6(4):1490-1491. doi: 10.1080/23802359.2020.1847611.

Abstract

var. is a well-regarded ornamental plant in the genus (family Iridaceae). In this report, we present the complete chloroplast (cp) genome sequence of var. for the first time. The complete cp genome of var. was assembled using high-throughput sequencing, and phylogenetic analysis was undertaken based on a dataset of coding regions. The cp genome of var. measures 152,409 bp in length, with regions having two inverted copies (IR 26,026 bp), and separated by the large single copy (LSC 82,256 bp) and small single copy (SSC 18,101 bp) regions. The cp genome encodes 133 unique genes, including 87 different protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Based on a dataset of 69 chloroplast coding regions, the maximum-likelihood (ML) phylogenetic tree analysis indicated that var. clusters closely with . Thus, the complete chloroplast genome presented in this report may provide valuable genetic information not only for the future exploitation and utilization of this plant resource but also for further research investigating its relationship with other species.

摘要

变种是鸢尾科鸢尾属中一种备受赞誉的观赏植物。在本报告中,我们首次展示了变种的完整叶绿体(cp)基因组序列。变种的完整cp基因组通过高通量测序进行组装,并基于编码区数据集进行系统发育分析。变种的cp基因组长度为152,409 bp,具有两个反向重复区域(IR为26,026 bp),并由大单拷贝(LSC为82,256 bp)和小单拷贝(SSC为18,101 bp)区域隔开。cp基因组编码133个独特基因,包括87个不同的蛋白质编码基因、38个tRNA基因和8个rRNA基因。基于69个叶绿体编码区的数据集,最大似然(ML)系统发育树分析表明变种与紧密聚类。因此,本报告中呈现的完整叶绿体基因组不仅可为该植物资源的未来开发和利用提供有价值的遗传信息,也可为进一步研究其与其他鸢尾属物种的关系提供帮助。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7eb/8081313/b98cb917d829/TMDN_A_1847611_F0001_B.jpg

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