断奶应激期间不同日粮叶酸水平对仔猪的影响。
Effects of varying dietary folic acid during weaning stress of piglets.
作者信息
Wang Lei, Tan Xian, Wang Huiru, Wang Qiye, Huang Pengfei, Li Yali, Li Jianzhong, Huang Jing, Yang Huansheng, Yin Yulong
机构信息
Laboratory of Animal Nutrition and Human Health, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Science, Hunan Normal University, Changsha, 410081, China.
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in Subtropical Region, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China.
出版信息
Anim Nutr. 2021 Mar;7(1):101-110. doi: 10.1016/j.aninu.2020.12.002. Epub 2020 Dec 23.
The present study was conducted to evaluate the effect of dietary folic acid on the growth performance, intestinal morphology, and intestinal epithelial cells renewal in post-weaning piglets. Twenty-eight piglets (weaned at day 21, initial body weight of 6.73 ± 0.62 kg) were randomly allotted to 4 treatments with 7 pens per diet and 1 piglet per pen. The piglets were fed the same antibiotic-free and zinc oxide-free basal diets supplemented with folic acid at 0, 3, 9, and 18 mg/kg for 14 days. The results showed that dietary supplementation with folic acid increased villus height (VH) ( = 0.003; linear, = 0.001), VH-to-crypt depth (VH:CD) ratio ( = 0.002; linear, = 0.001), villus surface area (VSA) ( = 0.026; linear, = 0.010). The analyzed parameters ADG, serum urea nitrogen (BUN) content, VH, VSA, and serum folate (SF) concentration responded linearly to the dietary folic acid concentration when the dietary folic acid concentration was below 4.42, 5.26, 4.79, 3.47, and 3.53 mg/kg respectively ( = 0.995, 0.995, 0.999, 0.999, 0.872, = 0.09, 0.07, 0.09, 0.09, 0.36, respectively), as assessed by a two-linear broken-line regression. Above these breakpoints, the response of ADG, VH, VSA, and SF plateaued in response to changes in dietary folic acid concentration. Moreover, dietary supplementation with folic acid significantly increased the lactase ( = 0.001; linear, = 0.001) and sucrase activities ( = 0.021; linear, = 0.010) in the jejunal mucosa of weaned piglets. The mRNA expression of solute carrier family 6 member 19 (), solute carrier family 1 member 1 (), tumor necrosis factor-α (), the number of Ki67 positive cells, and cell shedding rate had a significant linear contrast ( = 0.023, 0.021, 0.038, 0.049, and 0.008, respectively) in dietary folic acid groups. In conclusion, our results indicate that folic acid supplementation can improve the growth performance and intestinal morphology of weaned piglets by maintaining the balance of epithelial cell renewal.
本研究旨在评估日粮叶酸对断奶仔猪生长性能、肠道形态及肠上皮细胞更新的影响。选取28头仔猪（21日龄断奶，初始体重6.73±0.62千克），随机分为4组，每组7个重复栏，每栏1头仔猪。仔猪饲喂相同的无抗生素、无氧化锌基础日粮，分别添加0、3、9和18毫克/千克叶酸，为期14天。结果表明，日粮添加叶酸可增加绒毛高度（VH）（P = 0.003；线性关系，P = 0.001）、VH与隐窝深度（VH:CD）比值（P = 0.002；线性关系，P = 0.001）、绒毛表面积（VSA）（P = 0.026；线性关系，P = 0.010）。当日粮叶酸浓度分别低于4.42、5.26、4.79、3.47和3.53毫克/千克时，分析的参数平均日增重（ADG）、血清尿素氮（BUN）含量、VH、VSA和血清叶酸（SF）浓度对日粮叶酸浓度呈线性响应（R²分别为0.995、0.995、0.999、0.999、0.872，P分别为0.09、0.07、0.09、0.09、0.36），通过双线性折线回归评估。高于这些断点，ADG、VH、VSA和SF对日粮叶酸浓度变化的响应趋于平稳。此外，日粮添加叶酸显著提高了断奶仔猪空肠黏膜中乳糖酶（P = 0.001；线性关系，P = 0.001）和蔗糖酶活性（P = 0.021；线性关系，P = 0.010）。溶质载体家族6成员19（SLC6A19）、溶质载体家族1成员1（SLC1A1）、肿瘤坏死因子-α（TNF-α）的mRNA表达、Ki67阳性细胞数量和细胞脱落率在日粮叶酸组中有显著线性差异（P分别为0.023、0.021、0.038、0.049和0.008）。总之，我们的结果表明，补充叶酸可通过维持上皮细胞更新平衡来改善断奶仔猪的生长性能和肠道形态。
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