Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, China.
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
J Anim Physiol Anim Nutr (Berl). 2021 Mar;105(2):272-285. doi: 10.1111/jpn.13491. Epub 2021 Jan 5.
Fifty-six piglets were weaned at 21 days and randomly assigned to 1 of 8 dietary treatments with 7 replicate pens for a 14-day experimental period. The eight experimental diets were prepared via a 2 × 4 factorial arrangement with citric acid (CA; 0 and 0.3%) and dietary electrolyte balance (dEB, Na +K - Cl mEq/kg of the diet; -50, 100, 250, and 400 mEq/kg). Varying dEB values were obtained by altering calcium chloride and sodium bicarbonate contents. Dietary CA significantly increased (p < .05) villus height (VH) and villus height:crypt depth (VH:CD) in the jejunum. Piglets fed a 250 mEq/kg diet increased (p < .05) VH and VH:CD values in the duodenum. Jejunal VH and VH:CD increased (quadratic; p < .05), and ileal VH:CD (liner and quadratic; p < .05) decreased as dEB was increased in diets without CA, but no such effect was observed on the diets containing CA (dEB ×CA; p < .05). The CD in jejunum (quadratic; p < .05) increased as dEB was increased in diets containing CA, whereas it was decreased (linear; p < .05) in the diets without CA (dEB ×CA; p < .001). Dietary CA increased maltase activity and reduced the number of Ki67-positive cells (p < .05). Increasing dEB values in diets without CA increased sucrose and lactase activities (quadratic; p < .05), but no such effect was observed in the diets with CA (dEB ×CA; p < .05). An interaction effect between dEB and CA on the number of Ki67-positive cells was observed (p < .001). In conclusion, 250 mEq/kg dEB diet with CA improved piglet intestinal digestion and absorption function by improving intestinal morphology and increasing digestive enzyme activities. However, these improvements were also observed in piglets fed the 100 mEq/kg dEB diet without CA.
56 头仔猪在 21 日龄断奶，随机分为 8 种饮食处理组，每组 7 个重复栏，进行为期 14 天的实验。这 8 种实验日粮是通过柠檬酸（CA；0 和 0.3%）和日粮电解质平衡（dEB，饲料中的 Na +K - Cl mEq/kg；-50、100、250 和 400 mEq/kg）的 2×4 因子设计来制备的。通过改变氯化钙和碳酸氢钠的含量来获得不同的 dEB 值。日粮 CA 显著增加（p<0.05）了空肠的绒毛高度（VH）和绒毛高度：隐窝深度（VH：CD）。饲喂 250 mEq/kg 日粮的仔猪增加了（p<0.05）十二指肠的 VH 和 VH：CD 值。在不含 CA 的日粮中，随着 dEB 的增加，空肠的 VH 和 VH：CD 增加（二次；p<0.05），回肠的 VH：CD 减少（线性和二次；p<0.05），但在含有 CA 的日粮中没有观察到这种影响（dEB×CA；p<0.05）。在含有 CA 的日粮中，空肠的 CD 随 dEB 的增加而增加（二次；p<0.05），而在不含 CA 的日粮中则减少（线性；p<0.05）（dEB×CA；p<0.001）。日粮 CA 增加了麦芽糖酶的活性，减少了 Ki67 阳性细胞的数量（p<0.05）。在不含 CA 的日粮中，随着 dEB 值的增加，蔗糖和乳糖酶的活性增加（二次；p<0.05），但在含有 CA 的日粮中没有观察到这种影响（dEB×CA；p<0.05）。在 dEB 和 CA 对 Ki67 阳性细胞数量的影响之间观察到了互作效应（p<0.001）。总之，含 CA 的 250 mEq/kg dEB 日粮通过改善肠道形态和增加消化酶活性来提高仔猪的肠道消化吸收功能。然而，在仔猪饲喂不含 CA 的 100 mEq/kg dEB 日粮时，也观察到了这些改善。
