Shah Priyali, Wolever Thomas Ms, Jenkins Alexandra L, Ezatagha Adish, Campbell Janice, Zurbau Andreea, Jain Manish, Gote Manoj, Bhaduri Anirban, Mullick Ashim
Tata Chemicals Ltd-Innovation Centre, Pune, Maharashtra, India.
INQUIS Clinical Research (formerly GI Labs), Canada.
Heliyon. 2021 Apr 28;7(4):e06805. doi: 10.1016/j.heliyon.2021.e06805. eCollection 2021 Apr.
Short chain fructo-oligosaccharides (scFOS) are well-recognized prebiotic fibers. Fossence™ (FOSS) is a scFOS that has been produced from sucrose via a proprietary fermentation process and has not been tested for its digestibility or glucose/insulin response (GR and IR, respectively). The present randomized, controlled, cross-over study was conducted in 3 phases to explore GR and IR to ingestion of FOSS, when replaced by/added to available-carbohydrates (avCHO) among 25 healthy adults (40 ± 14years). In each phase GR and IR elicited by 3-4 test-meals were measured among the fasted recruited subjects. The interventional test meals were as follows: Phase-1, water alone or 10g FOSS or 10g Dextrose in 250ml water; Phase-2, 250ml water containing Dextrose:FOSS (g:g) in the content as 50:0 or 50:15 or 35:0 or 35:15; Phase-3 portions of white-bread (WB) containing avCHO:FOSS (g:g) in the content as 50:0 or 50:15 or 35:0 or 35:15. Blood samples (finger prick method) were collected at fasting and 15, 30, 45, 60, 90 and 120 min after start of test meal ingestion. Plasma glucose and serum insulin were analyzed utilizing standard methods. The primary endpoint was differences in glucose IAUC. All subjects provided their written consent to participate in the study (ClinicalTrials.gov: NCT03755232). The results demonstrated that FOSS, when consumed alone, showed no raise in glycaemia or insulinemia and was statistically equivalent to response of water alone. GR and IR elicited by dextrose:FOSS and WB:FOSS test-meals of Phase 2 and Phase 3, were statistically equivalent to the respective test-meals without FOSS. Result of the 3 phases support the hypothesis that FOSS is resistant to breakdown and is indigestible in the human small-intestine, and therefore can be classified as an unavailable carbohydrate that does not raise post prandial blood glucose or insulin. FOSS, being sweet to taste, may be an acceptable sugar replacer in beverages without compromising their taste and sensory qualities.
短链低聚果糖（scFOS）是公认的益生元纤维。Fossence™（FOSS）是一种通过专有发酵工艺由蔗糖生产的短链低聚果糖，尚未对其消化率或葡萄糖/胰岛素反应（分别为GR和IR）进行测试。本随机对照交叉研究分三个阶段进行，以探讨25名健康成年人（40±14岁）在将FOSS替代/添加到可利用碳水化合物（avCHO）中时，摄入FOSS后的GR和IR。在每个阶段，对空腹招募的受试者测量3 - 4次测试餐引发的GR和IR。干预性测试餐如下：第一阶段，单独饮用250毫升水或10克FOSS或10克葡萄糖；第二阶段，250毫升水中含有葡萄糖:FOSS（克:克）比例为50:0或50:15或35:0或35:15；第三阶段，白面包（WB）中含有avCHO:FOSS（克:克）比例为50:0或50:15或35:0或35:15。在空腹以及摄入测试餐开始后的15、30、45、60、90和120分钟采集血样（手指针刺法）。使用标准方法分析血浆葡萄糖和血清胰岛素。主要终点是葡萄糖IAUC的差异。所有受试者均书面同意参与该研究（ClinicalTrials.gov: NCT03755232）。结果表明，单独食用FOSS时，血糖和胰岛素水平没有升高，在统计学上与单独饮水的反应相当。第二阶段和第三阶段葡萄糖:FOSS和WB:FOSS测试餐引发的GR和IR在统计学上与不含FOSS的相应测试餐相当。三个阶段的结果支持了以下假设：FOSS在人体小肠中抗分解且不可消化，因此可归类为不会升高餐后血糖或胰岛素的不可利用碳水化合物。FOSS味道甜美，在不影响饮料口感和感官品质的情况下，可能是一种可接受的糖替代品。
