Fattahi Sadegh, Nikbakhsh Novin, Taheri Hassan, Ghadami Elham, Ranaee Mohammad, Akhavan-Niaki Haleh
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Noncoding RNA Res. 2021 Apr 21;6(2):86-91. doi: 10.1016/j.ncrna.2021.04.002. eCollection 2021 Jun.
Despite considerable progress in gastric cancer screening, prevention, and treatment, it remains a major cause of morbidity and mortality worldwide. Due to late diagnosis of the disease, early potential diagnostic biomarkers are needed. Accumulating evidence indicates that non-coding RNAs have potential applications as diagnostic and prognostic biomarkers in gastric cancer. Herein, we investigated the expression levels of two novel non-coding RNAs, long intergenic non-protein coding RNA 2688 () and () by real-time PCR for the first time in 47 gastric cancer patients. We found significant downregulation of LINC02688 and () with 3.44 and 2.2-fold decrease, respectively in tumoral tissues in comparison with their adjacent non-tumoral counterparts (P < 0.0001). Our data also indicates that more than 96% and 88% of patients showed unchanged or decreased expression of LINC02688 and (), respectively. As most gastric cancer patients showed lower expression of these two lncRNAs, no significant association between clinicopathological features of the patients and the level of LINC02688 and () expression could be detected. Furthermore, ROC curve analysis indicated that LINC02688 and can serve as good predictive biomarkers for distinguishing tumoral tissues from their adjacent non-tumoral counterparts. Taken together, our findings suggested that these two novel tumor suppressor non-coding RNAs may act as novel diagnostic biomarkers for diagnosis of carcinogenesis event even at earlier stages of gastric adenocarcinoma.
尽管在胃癌筛查、预防和治疗方面取得了显著进展，但它仍是全球发病和死亡的主要原因。由于该疾病诊断较晚，因此需要早期潜在的诊断生物标志物。越来越多的证据表明，非编码RNA在胃癌中作为诊断和预后生物标志物具有潜在应用价值。在此，我们首次通过实时PCR检测了47例胃癌患者中两种新型非编码RNA，即长链基因间非编码RNA 2688（LINC02688）和[具体名称缺失]的表达水平。我们发现，与相邻的非肿瘤组织相比，肿瘤组织中LINC02688和[具体名称缺失]显著下调，分别下降了3.44倍和2.2倍（P < 0.0001）。我们的数据还表明，分别有超过96%和88%的患者LINC02688和[具体名称缺失]的表达未改变或下降。由于大多数胃癌患者这两种长链非编码RNA表达较低，因此未检测到患者临床病理特征与LINC02688和[具体名称缺失]表达水平之间存在显著关联。此外，ROC曲线分析表明，LINC02688和[具体名称缺失]可作为区分肿瘤组织与其相邻非肿瘤组织的良好预测生物标志物。综上所述，我们的研究结果表明，这两种新型肿瘤抑制性非编码RNA可能作为新型诊断生物标志物，用于诊断胃癌发生事件，甚至在胃腺癌的早期阶段。
