Mota Raquel, Pinto Marisa, Palmeira Josman, Gonçalves Daniela, Ferreira Helena
UCIBIO, Microbiology, Faculty of Pharmacy of University of Porto, Portugal.
Microbiology, Faculty of Pharmacy of University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Access Microbiol. 2020 Nov 25;3(1):acmi000182. doi: 10.1099/acmi.0.000182. eCollection 2021.
Multidrug-resistant bacteria have been increasingly described in healthcare institutions, however community resistance also seems to be emerging. an intestinal commensal bacteria, is also a pathogen and represents an important intestinal reservoir of resistance. Our aim was the study of the intestinal colonization and of the persistence of antibiotic resistant intestinal bacteria in healthy university students of Porto, in the north of Portugal. Samples from 30 university students were collected and analysed. Two isolates were randomly obtained from each student and Gram-negative bacilli resistant to antibiotics were studied. In addition, we evaluated changes in the Gram-negative intestinal colonization of ten university students in a short period of time. Molecular characterization showed a high presence of in commensal . Gram-negative bacteria with intrinsic and extrinsic resistance were isolated, namely spp., spp. and spp. We isolated three ESBL-producing from two students. These isolates showed group 1 (=1), group 9 (=2), (=2), (=1) and (=2) genes. Additionally, they showed specific virulence factors and conjugational transfer of antibiotic resistance and virulence genes. One spp. isolate resistant to carbapenems was detected colonizing one student. Our results confirm that healthy young adults may be colonized with commensals showing clinically relevant antibiotic resistance mechanisms, creating a risk of silent spread of these bacteria in the community.
医疗机构中对多种药物耐药的细菌越来越多地被发现，然而社区耐药情况似乎也在出现。作为一种肠道共生菌，同时也是一种病原体，是耐药性的重要肠道储存库。我们的目的是研究葡萄牙北部波尔图市健康大学生肠道中抗生素耐药性肠道细菌的定植和持续存在情况。收集并分析了30名大学生的样本。从每个学生中随机获取两个分离株，并对耐抗生素的革兰氏阴性杆菌进行研究。此外，我们在短时间内评估了10名大学生革兰氏阴性肠道定植的变化。分子特征表明共生菌中存在大量[具体细菌名称未给出]。分离出了具有内在和外在耐药性的革兰氏阴性细菌，即[具体细菌种类未给出]菌属、[具体细菌种类未给出]菌属和[具体细菌种类未给出]菌属。我们从两名学生中分离出了三株产超广谱β-内酰胺酶（ESBL）的[具体细菌名称未给出]。这些分离株显示出1型[具体基因名称未给出]群（=1）、9型[具体基因名称未给出]群（=2）、[具体基因名称未给出]（=2）、[具体基因名称未给出]（=1）和[具体基因名称未给出]（=2）基因。此外，它们还显示出特定的毒力因子以及抗生素耐药性和毒力基因的接合转移。检测到一名学生的肠道中定植了一株对碳青霉烯类耐药的[具体细菌名称未给出]菌属分离株。我们的结果证实，健康的年轻人可能被具有临床相关抗生素耐药机制的共生菌定植，从而在社区中造成这些细菌无声传播的风险。
