Department of Physiology, Faculty of Veterinary, University of Balıkesir, Balıkesir, Turkey.
Department of Histology and Embryology, Faculty of Veterinary, University of Balıkesir, Balıkesir, Turkey.
Biotech Histochem. 2022 Apr;97(3):192-198. doi: 10.1080/10520295.2021.1925965. Epub 2021 May 17.
We investigated the effects of melatonin (Mlt) on hematological characteristics to determine the percentage of alpha-naphthyl acetate esterase (ANAE) positive lymphocytes in rats chronically exposed to cadmium (Cd). We used four groups of male rats: control (C), melatonin (Mlt), cadmium (Cd) and Cd + Mlt (CdMlt). Blood samples were obtained to assess the erythrocytes (RBC), leukocytes (WBC), platelets (PLT), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV) as well as alpha-naphthyl acetate esterase (ANAE) positive lymphocytes. We found that the number of WBC increased in the Cd group compared to the other three experimental groups. Low MCV and MCHC values caused by Cd were ameliorated by Mlt treatment in the CdMlt group. We found no significant change in the RBC, HGB, MCH, PLT and HCT values among the experimental groups. Mlt treatment also increased the percentage of ANAE positive lymphocytes. Mlt partially protects some hematological characteristics in cases of chronic Cd toxicity.
我们研究了褪黑素（Mlt）对血液特征的影响，以确定长期接触镉（Cd）的大鼠中α-醋酸萘酯酶（ANAE）阳性淋巴细胞的百分比。我们使用了四组雄性大鼠：对照组（C）、褪黑素（Mlt）组、镉（Cd）组和 Cd+褪黑素（CdMlt）组。采集血液样本以评估红细胞（RBC）、白细胞（WBC）、血小板（PLT）、血红蛋白（HGB）、血细胞比容（HCT）、平均红细胞血红蛋白（MCH）、平均红细胞血红蛋白浓度（MCHC）、平均红细胞体积（MCV）以及α-醋酸萘酯酶（ANAE）阳性淋巴细胞。我们发现，与其他三组实验相比，Cd 组的 WBC 数量增加。在 CdMlt 组中，Mlt 治疗改善了由 Cd 引起的低 MCV 和低 MCHC 值。我们在实验组之间未发现 RBC、HGB、MCH、PLT 和 HCT 值的显著变化。Mlt 治疗还增加了 ANAE 阳性淋巴细胞的百分比。Mlt 部分保护了慢性 Cd 毒性情况下的一些血液特征。
