Chair of Neuronal Control and Metabolism, School of Life Sciences, Technical University of Munich.
Chair of Neuronal Control and Metabolism, School of Life Sciences, Technical University of Munich;
J Vis Exp. 2021 Apr 27(170). doi: 10.3791/61876.
We present a method developed specifically to image the whole Drosophila brain during ongoing behavior such as walking. Head fixation and dissection are optimized to minimize their impact on behavior. This is first achieved by using a holder that minimizes movement hindrances. The back of the fly's head is glued to this holder at an angle that allows optical access to the whole brain while retaining the fly's ability to walk, groom, smell, taste and see. The back of the head is dissected to remove tissues in the optical path and muscles responsible for head movement artefacts. The fly brain can subsequently be imaged to record brain activity, for instance using calcium or voltage indicators, during specific behaviors such as walking or grooming, and in response to different stimuli. Once the challenging dissection, which requires considerable practice, has been mastered, this technique allows to record rich data sets relating whole brain activity to behavior and stimulus responses.
我们提出了一种专门用于在行走等持续行为过程中对整个果蝇大脑进行成像的方法。头部固定和解剖经过优化，以最大程度地减少它们对行为的影响。这首先是通过使用一种支架来实现的，这种支架最小化了运动障碍。将果蝇头部的背面以一定角度粘贴到该支架上，该角度允许对整个大脑进行光学访问，同时保持果蝇行走、梳理、嗅觉、味觉和视觉的能力。头部的背面被解剖以去除光学路径中的组织和负责头部运动伪影的肌肉。随后可以对果蝇大脑进行成像，以在特定行为（例如行走或梳理）期间以及对不同刺激的反应过程中记录大脑活动，例如使用钙或电压指示剂。一旦掌握了具有挑战性的解剖技术（需要大量实践），该技术就可以记录与行为和刺激反应相关的丰富数据集。
