School of Life Sciences, Technical University of Munich, Freising, Germany.
University of Bonn, Medical Faculty (UKB), Institute of Physiology II, Bonn, Germany.
Elife. 2023 Apr 17;12:e85202. doi: 10.7554/eLife.85202.
Movement-correlated brain activity has been found across species and brain regions. Here, we used fast whole brain lightfield imaging in adult to investigate the relationship between walk and brain-wide neuronal activity. We observed a global change in activity that tightly correlated with spontaneous bouts of walk. While imaging specific sets of excitatory, inhibitory, and neuromodulatory neurons highlighted their joint contribution, spatial heterogeneity in walk- and turning-induced activity allowed parsing unique responses from subregions and sometimes individual candidate neurons. For example, previously uncharacterized serotonergic neurons were inhibited during walk. While activity onset in some areas preceded walk onset exclusively in spontaneously walking animals, spontaneous and forced walk elicited similar activity in most brain regions. These data suggest a major contribution of walk and walk-related sensory or proprioceptive information to global activity of all major neuronal classes.
已经在不同物种和脑区发现了与运动相关的大脑活动。在这里，我们使用快速全脑光场成像技术在成年中研究行走与全脑神经元活动之间的关系。我们观察到与自发行走密切相关的活动整体变化。在对特定的兴奋性、抑制性和神经调质神经元进行成像时，突出了它们的共同贡献，而行走和转弯引起的活动的空间异质性允许从小脑区域和有时是单个候选神经元中解析出独特的反应。例如，以前未被描述的血清素能神经元在行走时被抑制。虽然一些区域的活动起始时间仅在自发行走的动物中早于行走起始时间，但自发和强制行走在大多数脑区引起相似的活动。这些数据表明，行走和与行走相关的感觉或本体感觉信息对所有主要神经元类别的整体活动有很大的贡献。
