Sudalaimani S, Sanjeev Kumar K, Esokkiya A, Suresh C, Giribabu K
Electrodics and Electrocatalysis Division, CSIR-Central Electrochemical Research Institute, Karaikudi-630 003, Tamil Nadu, India.
Analyst. 2021 May 21;146(10):3208-3215. doi: 10.1039/d1an00019e. Epub 2021 Apr 7.
Putrescine and cadaverine are biogenic amines that serve as potential biomarkers for several types of cancers and monitoring food quality. Electrochemical sensing of putrescine and cadaverine by non-enzymatic routes remains a challenge because of their inertness at unmodified electrode surfaces and hence a liquid-liquid interface strategy has been employed for their detection. In the present study, electrochemical sensing of cadaverine and putrescine has been demonstrated by simple and facilitated ion-transfer processes using a liquid-liquid microinterface supported by a microcapillary. A microinterface was constructed in different configurations by varying the aqueous phase composition in the absence and presence of dibenzo-18-crown-6, and the ion-transfer ability of putrescine and cadaverine was studied in these configurations. A peak shaped voltammogram was observed in the backward scan, due to the linear diffusion of putrescine and cadaverine from the organic to the aqueous phase. The detection ability in the presence of dibenzo-18-crown-6 was observed in the concentration ranges of 0.25-25 μM and 0.25-40 μM for putrescine and cadaverine with detection limits of 0.11 and 0.17 μM respectively. In the presence of dibenzo-18-crown-6, the electrochemical sensing of putrescine and cadaverine was more pronounced compared to the simple ion-transfer process.
腐胺和尸胺是生物胺，可作为多种癌症的潜在生物标志物并用于监测食品质量。通过非酶途径对腐胺和尸胺进行电化学传感仍然是一项挑战，因为它们在未修饰的电极表面具有惰性，因此采用了液-液界面策略对其进行检测。在本研究中，通过使用微毛细管支撑的液-液微界面的简单且便利的离子转移过程，实现了对尸胺和腐胺的电化学传感。通过在有无二苯并-18-冠-6的情况下改变水相组成，构建了不同构型的微界面，并研究了腐胺和尸胺在这些构型中的离子转移能力。由于腐胺和尸胺从有机相到水相的线性扩散，在反向扫描中观察到了峰形伏安图。在存在二苯并-18-冠-6的情况下，腐胺和尸胺的检测浓度范围分别为0.25 - 25 μM和0.25 - 40 μM，检测限分别为0.11和0.17 μM。与简单的离子转移过程相比，在存在二苯并-18-冠-6的情况下，腐胺和尸胺的电化学传感更为明显。
