Biset Sirak, Benti Ayantu, Molla Lidya, Yimer Sitot, Cherkos Tena, Eyayu Yalewayker, Ebabu Ashenafi, Kasew Desie, Ambachew Aklilu
Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, University of Gondar, Gondar, Ethiopia.
School of Biomedical and Laboratory Sciences, University of Gondar, Gondar, Ethiopia.
Infect Drug Resist. 2021 May 10;14:1703-1711. doi: 10.2147/IDR.S307156. eCollection 2021.
Meningitis, which is mostly caused by infectious agents, occurs more commonly during the first month of life. Bacterial meningitis is an important source of mortality and morbidity, especially in neonates of resource-limited countries. We aimed to determine the prevalence and etiological agents of bacterial meningitis and their antibiotic susceptibility pattern in neonates at the University of Gondar Comprehensive Specialized Hospital.
We collected retrospective data from bacteriological results of cerebrospinal fluid of meningitis suspected neonates from 2013 to 2019. Sample collection, culture preparation, bacterial identification, and susceptibility testing were performed using standard microbiological techniques. We extracted data on socio-demographic characteristics and culture and antibiotic susceptibility testing results. We inputted the data using Epi-info version 7 and exported it to SPSS version 20 for analysis.
In this study, 1101 cerebrospinal fluid samples, 595 (54%) male and 506 (46%) female neonates, were cultured to look for meningitis-causing bacteria. Of 1101 cerebrospinal fluid, 19 (1.73%) were culture positive for meningitis-causing bacteria. The common etiological agents were 36.8% (7), non-lactose-fermenter Gram-negative rods 21% (4), and Group B streptococcus 15.8% (3). The overall resistance rate among cephalosporin, cotrimoxazole, penicillin, and aminoglycosides classes were 90%, 88.9%, 77.3%, and 54.54%, respectively. Of all isolates, 58% (11) were multidrug-resistant, including all the non-lactose-fermenter Gram-negative rods and 71.4% of the isolates.
The prevalence of neonatal bacterial meningitis was 1.73%. and other Gram-negative rods, with a high multidrug-resistant rate, were the leading cause of neonatal bacterial meningitis. Further studies are needed to explore the source of infection, incidence, and risk factors of neonatal bacterial meningitis.
脑膜炎大多由感染因子引起，在出生后的第一个月更为常见。细菌性脑膜炎是导致死亡和发病的重要原因，在资源有限国家的新生儿中尤其如此。我们旨在确定贡德尔大学综合专科医院新生儿细菌性脑膜炎的患病率、病原体及其抗生素敏感性模式。
我们收集了2013年至2019年疑似脑膜炎新生儿脑脊液细菌学检查结果的回顾性数据。样本采集、培养准备、细菌鉴定和药敏试验均采用标准微生物学技术。我们提取了社会人口学特征以及培养和抗生素药敏试验结果的数据。我们使用Epi-info 7版输入数据，并将其导出到SPSS 20版进行分析。
在本研究中，共培养了1101份脑脊液样本，其中595例（54%）为男婴，506例（46%）为女婴，以寻找引起脑膜炎的细菌。在1101份脑脊液样本中，19份（1.73%）培养出引起脑膜炎的细菌呈阳性。常见病原体为非乳糖发酵革兰氏阴性杆菌占36.8%（7例）、21%（4例），B组链球菌占15.8%（3例）。头孢菌素类、复方新诺明、青霉素类和氨基糖苷类的总体耐药率分别为90%、88.9%、77.3%和54.54%。在所有分离株中，58%（11例）为多重耐药，包括所有非乳糖发酵革兰氏阴性杆菌和71.4%的分离株。
新生儿细菌性脑膜炎的患病率为1.73%。非乳糖发酵革兰氏阴性杆菌等革兰氏阴性杆菌，多重耐药率高，是新生儿细菌性脑膜炎的主要原因。需要进一步研究以探索新生儿细菌性脑膜炎的感染源、发病率和危险因素。
