人脂肪来源基质细胞的单细胞转录组分析鉴定出一种收缩性细胞亚群。
Single-Cell Transcriptome Analysis of Human Adipose-Derived Stromal Cells Identifies a Contractile Cell Subpopulation.
作者信息
Wolmarans Elize, Mellet Juanita, Durandt Chrisna, Joubert Fourie, Pepper Michael S
机构信息
Institute for Cellular and Molecular Medicine, Department of Immunology, SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa.
Centre for Bioinformatics and Computational Biology & Genomics Research Institute, University of Pretoria, Pretoria 0001, South Africa.
出版信息
Stem Cells Int. 2021 Apr 28;2021:5595172. doi: 10.1155/2021/5595172. eCollection 2021.
The potential for human adipose-derived stromal cells (hASCs) to be used as a therapeutic product is being assessed in multiple clinical trials. However, much is still to be learned about these cells before they can be used with confidence in the clinical setting. An inherent characteristic of hASCs that is not well understood is their heterogeneity. The aim of this exploratory study was to characterize the heterogeneity of freshly isolated hASCs after two population doublings (P2) using single-cell transcriptome analysis. A minimum of two subpopulations were identified at P2. A major subpopulation was identified as contractile cells which, based on gene expression patterns, are likely to be pericytes and/or vascular smooth muscle cells (vSMCs).
目前多项临床试验正在评估人脂肪来源的基质细胞（hASC）用作治疗产品的潜力。然而，在能够放心地将这些细胞用于临床之前，仍有许多需要了解的地方。hASC的一个尚未被充分理解的固有特征是它们的异质性。这项探索性研究的目的是通过单细胞转录组分析来表征经过两次群体倍增（P2）后新鲜分离的hASC的异质性。在P2阶段至少鉴定出两个亚群。一个主要亚群被鉴定为收缩性细胞，根据基因表达模式，它们可能是周细胞和/或血管平滑肌细胞（vSMC）。
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