Sayad Arezou, Hashemian Fatemeh, Gholami Leila, Jamali Masoud, Badrlou Elham, Sadeghpour Saba, Nazer Naghme, Khalilian Sheyda, Ghafouri-Fard Soudeh
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Periodontics, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
BMC Res Notes. 2025 May 12;18(1):211. doi: 10.1186/s13104-025-07274-4.
This study aims at determination of the roles of five apoptosis-related genes, namely CASP2, CASP8, BCL2, HULC and PVT1 in the pathoetiology of periodontitis via measurement of their expressions in both peripheral blood and tissues of affected persons.
CASP2 was over-expressed in gingiva of patients compared with healthy subjects (RME = 24.56, P < 0.001), and in both affected females and males (RME = 30.53, P = 0.03 and RME = 20.59, P = 0.01, respectively). BCL2 was higher in affected tissues compared with controls (RME = 32.28, P < 0.001) and in affected tissues of males versus controls (RME = 69.03, P < 0.001). Finally, HULC had lower level in the blood of patients (RME = 0.21, P = 0.01) and in the blood of female patients compared with normal females (RME = 0.15, P = 0.01). Other comparisons yielded no significant results. BCL2 and CASP2 had the highest diagnostic values for separation of diseased gingival tissues from normal ones. HULC has the best values in the distinction of blood samples of affected persons from control persons. Combination of transcript levels of CASP2, CASP8, BCL2, HULC and PVT1 changed AUC to 0.84 and 0.72 in tissues and blood samples, respectively. To conclude, these genes might be regarded as putative contributors in the pathophysiology of periodontitis.
本研究旨在通过检测五个凋亡相关基因，即半胱天冬酶2（CASP2）、半胱天冬酶8（CASP8）、B细胞淋巴瘤-2（BCL2）、长链非编码RNA HULC（HULC）和 Pvt1基因座（PVT1）在牙周炎患者外周血和组织中的表达，来确定它们在牙周炎病理病因中的作用。
与健康受试者相比，CASP2在患者牙龈中过度表达（相对倍数变化[RME]=24.56，P<0.001），在患病女性和男性中均如此（RME分别为30.53，P=0.03和RME=20.59，P=0.01）。与对照组相比，BCL2在患病组织中表达较高（RME=32.28，P<0.001），在男性患病组织与对照组相比时更高（RME=69.03，P<0.001）。最后，与正常女性相比，HULC在患者血液中水平较低（RME=0.21，P=0.01），在女性患者血液中也较低（RME=0.15，P=0.01）。其他比较未产生显著结果。BCL2和CASP2在区分患病牙龈组织与正常组织方面具有最高的诊断价值。HULC在区分患者血液样本与对照者血液样本方面具有最佳价值。CASP2、CASP8、BCL2、HULC和PVT1的转录水平组合在组织和血液样本中的曲线下面积（AUC）分别变为0.84和0.72。总之，这些基因可能被视为牙周炎病理生理学中的潜在因素。
