Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
Centre of Excellence in Medical Biotechnology, Naresuan University, Phitsanulok 65000, Thailand.
Epigenomics. 2021 Jun;13(12):953-965. doi: 10.2217/epi-2020-0463. Epub 2021 May 19.
We investigated DNA methylation of in methamphetamine (METH) dependence in humans and an animal model. methylation at exon IV was determined by pyrosequencing of blood DNA from METH-dependent and control subjects, and from rat brain following an escalating dose of METH or vehicle. expression was determined in rat brain. methylation was increased in human METH dependence, greatest in subjects with psychosis and in prefrontal cortex of METH-administered rats; rat hippocampus showed reduced methylation and increased gene expression. methylation is abnormal in human METH dependence, especially METH-dependent psychosis, and in METH-administered rats. This may influence expression and contribute to the neurotoxic effects of METH exposure.
我们研究了人类和动物模型中甲基苯丙胺（METH）依赖的 DNA 甲基化。通过对依赖 METH 的人和对照组的血液 DNA 进行焦磷酸测序，以及对接受递增剂量 METH 或载体的大鼠脑进行检测，确定了外显子 IV 的甲基化。在大鼠脑中测定了表达。METH 依赖者的 DNA 甲基化增加，精神病患者和 METH 给药大鼠的前额叶皮质中增加最多；大鼠海马显示出减少的 甲基化和增加的基因表达。人类 METH 依赖，尤其是 METH 依赖型精神病，以及接受 METH 处理的大鼠中， 甲基化异常。这可能会影响 表达并导致 METH 暴露的神经毒性作用。
