Department of Comprehensive Dentistry, College of Dentistry, Texas A&M University, Dallas, TX, USA.
Department of Biomedical Sciences, College of Dentistry, Texas A&M University, Dallas, TX, USA.
J Dent Res. 2022 Jan;101(1):73-82. doi: 10.1177/00220345211013722. Epub 2021 May 19.
Osseointegration is the key issue for implant success. The in vivo properties of cell populations driving the osseointegration process have remained largely unknown. In the current study, using tissue clearing-based 3-dimensional imaging and transgenic mouse model-based lineage tracing methods, we identified Gli1+ cells within alveolar bone marrow and their progeny as the cell population participating in extraction socket healing and implant osseointegration. These Gli1 cells are surrounding blood vessels and do not express lineage differentiation markers. After tooth extraction and delayed placement of a dental implant, Gli1 cells were activated into proliferation, and their descendants contributed significantly to new bone formation. Ablation of Gli1 cells severely compromised the healing and osseointegration processes. Blockage of canonical Wnt signaling resulted in impaired recruitment of Gli1 cells and compromised bone healing surrounding implants. Collectively, these findings demonstrate that Gli1 cells surrounding alveolar bone marrow vasculature are stem cells supporting dental implant osseointegration. Canonical Wnt signal plays critical roles in regulating Gli1 stem cells.
骨整合是种植体成功的关键问题。在体内驱动骨整合过程的细胞群体的特性在很大程度上仍然未知。在本研究中，我们使用基于组织透明化的三维成像和基于转基因小鼠模型的谱系追踪方法，鉴定出牙槽骨骨髓中的Gli1+细胞及其后代是参与拔牙窝愈合和种植体骨整合的细胞群体。这些 Gli1 细胞位于血管周围，不表达谱系分化标志物。在拔牙和延迟放置牙种植体后，Gli1 细胞被激活进入增殖状态，其后代对新骨形成有重要贡献。Gli1 细胞的缺失严重损害了愈合和骨整合过程。阻断经典 Wnt 信号导致 Gli1 细胞的募集受损，并损害了种植体周围的骨愈合。总的来说，这些发现表明，牙槽骨骨髓血管周围的 Gli1 细胞是支持牙种植体骨整合的干细胞。经典 Wnt 信号在调节 Gli1 干细胞中发挥着关键作用。
