Kristensen S D, Roberts K M, Lawry J, Martin J F
Department of Medicine, University of Sheffield, Royal Hallamshire Hospital, U.K.
Atherosclerosis. 1988 Jun;71(2-3):121-30. doi: 10.1016/0021-9150(88)90136-0.
The effect of a short-term high cholesterol diet on thrombopoiesis and vascular ultrastructure was evaluated in rabbits. Six pairs of male litter-mate rabbits were randomized pairwise to feeding with either 2 g of cholesterol daily in addition to their normal diet or normal diet alone for 7 days. A significant 12-fold increase in median serum cholesterol (P less than 0.035) and an insignificant decrease in platelet count (P = 0.07) were found in the animals fed a high cholesterol diet. In these animals the total and cytoplasmic megakaryocyte size measured as planimetric areas in bone marrow sections were significantly decreased (P less than 0.035). No statistically significant difference in the megakaryocyte DNA content measured by flow cytometry in marrow suspensions enriched for megakaryocytes by density gradient centrifugation and centrifugal elutriation was observed between the cholesterol-fed animals and controls. Light microscopic, transmission and scanning electron microscopic examination of the aorta in both groups of animals showed a morphologically intact endothelium without any adhesion of blood-borne cells to the luminal surface. Transmission electron microscopic studies showed that cells with ultrastructural features resembling smooth muscle cells were present in the intima of the aortas of the animals on the high cholesterol diet, but not in control animals. A decrease in the size of bone marrow megakaryocytes and the occurrence of intimal smooth muscle cells are found in rabbits fed a high cholesterol diet for 7 days. These cellular events may be important features in early atherogenesis.
在兔中评估了短期高胆固醇饮食对血小板生成和血管超微结构的影响。将六对雄性同窝出生的兔随机两两分组，一组除正常饮食外每天喂2克胆固醇，另一组仅喂正常饮食，持续7天。喂食高胆固醇饮食的动物血清胆固醇中位数显著升高12倍（P<0.035），血小板计数有不显著下降（P=0.07）。在这些动物中，以骨髓切片中平面面积测量的总巨核细胞和细胞质巨核细胞大小显著减小（P<0.035）。通过密度梯度离心和离心淘洗富集巨核细胞的骨髓悬液，用流式细胞术测量巨核细胞DNA含量，在喂食胆固醇的动物和对照组之间未观察到统计学显著差异。两组动物主动脉的光镜、透射电镜和扫描电镜检查均显示内皮形态完整，管腔表面无血源性细胞黏附。透射电镜研究表明，高胆固醇饮食动物主动脉内膜存在具有类似平滑肌细胞超微结构特征的细胞，而对照动物中则没有。在喂食高胆固醇饮食7天的兔中发现骨髓巨核细胞大小减小以及内膜平滑肌细胞出现。这些细胞事件可能是早期动脉粥样硬化形成的重要特征。
