Martin J F, Slater D N, Kishk Y T, Trowbridge E A
Arteriosclerosis. 1985 Nov-Dec;5(6):604-12. doi: 10.1161/01.atv.5.6.604.
Rabbits were fed either 2 g cholesterol in 10 ml olive oil daily with normal diet (n = 5) or normal diet alone (n = 5). After 12 weeks, the cholesterol-fed animals had developed fatty plaques involving 24% +/- 4% of the surface area of the aorta; the control animals had none. Mean platelet volume was significantly smaller (p less than 0.04) in the cholesterol-fed animals (4.1 +/- 0.3 fl) compared with the controls (4.8 +/- 0.4 fl). The heterogeneity of the average volume distributions of the two groups, characterized by the statistical parameters of the coefficient of variation, skewness, and kurtosis, was also significantly different. Platelet count was significantly higher (p less than 0.001) in the cholesterol-fed group (7.48 +/- 1.06 x 10(11) platelets/liter blood) compared to the control group (4.86 +/- 0.60 x 10(11) platelets/liter blood). Mean megakaryocyte cytoplasmic volume was significantly larger (p less than 0.001) in the cholesterol-fed rabbits (12,262 +/- 1485 fl) compared with controls (6,814 +/- 761 fl). The range of cytoplasmic volumes was also significantly increased in the cholesterol-fed rabbits. A significant (p less than 0.01) increase in mean megakaryocyte nuclear volume in the cholesterol-fed animals was accompanied by a nonsignificant increase in mean nuclear DNA content: 30.2 +/- 3.7 N compared with a control value of 23.6 +/- 4.0 N. This evidence indicates that a high cholesterol diet in rabbits is associated with changes in platelet production from megakaryocytes as well with as the development of atherosclerosis.
给兔子每日喂食含2克胆固醇的10毫升橄榄油并搭配正常饮食（n = 5），或仅喂食正常饮食（n = 5）。12周后，喂食胆固醇的动物主动脉表面积出现了占24%±4%的脂肪斑块；而对照动物则没有。与对照组（4.8±0.4飞升）相比，喂食胆固醇的动物平均血小板体积显著更小（p<0.04）（4.1±0.3飞升）。以变异系数、偏度和峰度等统计参数为特征的两组平均体积分布的异质性也存在显著差异。与对照组（4.86±0.60×10¹¹个血小板/升血液）相比，喂食胆固醇组的血小板计数显著更高（p<0.001）（7.48±1.06×10¹¹个血小板/升血液）。与对照组（6814±761飞升）相比，喂食胆固醇的兔子平均巨核细胞胞质体积显著更大（p<0.001）（12262±1485飞升）。喂食胆固醇的兔子胞质体积范围也显著增加。喂食胆固醇的动物平均巨核细胞核体积显著增加（p<0.01），同时平均核DNA含量无显著增加：30.2±3.7N，而对照值为23.6±4.0N。这一证据表明，兔子的高胆固醇饮食与巨核细胞产生血小板的变化以及动脉粥样硬化的发展有关。
