Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA.
Department of Neuroscience, The Ohio State University, Columbus, OH 43210, USA.
STAR Protoc. 2021 May 3;2(2):100513. doi: 10.1016/j.xpro.2021.100513. eCollection 2021 Jun 18.
Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease. We recently developed scREAD, a database to provide comprehensive analyses of all the existing AD scRNA-seq and snRNA-seq data from the public domain. Here, we report protocols for using the scREAD web interface and running the backend workflow locally. Our protocols enable custom analyses of AD single-cell and single-nucleus gene expression profiles. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2020).
单细胞 RNA 测序 (scRNA-seq) 和单核 RNA 测序 (snRNA-seq) 研究为理解阿尔茨海默病的分子发病机制提供了重要的见解。我们最近开发了 scREAD，这是一个数据库，用于对来自公共领域的所有现有的 AD scRNA-seq 和 snRNA-seq 数据进行全面分析。在这里，我们报告了使用 scREAD 网络界面和在本地运行后端工作流程的协议。我们的协议允许对 AD 单细胞和单核基因表达谱进行自定义分析。有关此协议的使用和执行的完整详细信息，请参阅 Jiang 等人。（2020 年）。
