State Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.
Anal Bioanal Chem. 2021 Jul;413(16):4267-4275. doi: 10.1007/s00216-021-03378-5. Epub 2021 May 19.
In our investigation, a smartphone-integrated ratiometric fluorescent sensing system with lanthanide-based infinite coordination polymers (Ce-GMP-DPA@Tb-DPA) as signal probe has been successfully constructed for sensitive and portable detection of water in organic solvents. The Ce-GMP-DPA presents blue luminescence which is suppressed once the Tb-DPA is integrated to form the complex of Ce-GMP-DPA@Tb-DPA. Due to the energy transfer from Ce to Tb, the as-formed Ce-GMP-DPA@Tb-DPA exhibits green fluorescence of Tb-DPA. The presence of water can decompose the Tb-DPA, which blocks energy transfer from Ce to Tb, resulting in the decrease of green emission of Tb-DPA and the recovery of blue emission of Ce-GMP-DPA. Therefore, a ratiometric fluorescence assay is established for quantitative water detection within a wide linear range from 0.2 to 90.0% in ethanol. The limit of detection (LOD) reaches as low as 0.16% in ethanol, 0.62% in THF, and 0.0076% in acetonitrile, respectively. Furthermore, a smartphone installed with Color Picker APP as signal reader and analyzer is designed to integrate with the detection assay. With white spirit as real sample, water can be on-site detected with high accuracy (RSD < 2.81%). The developed platform presents great potential for portable water detection in practical application with merits of low cost, easy carry, and simple operation.
在我们的研究中，成功构建了一种基于智能手机的铕（III）-配位聚合物比率荧光传感系统，用于在有机溶剂中对水进行灵敏和便携的检测。Ce-GMP-DPA 呈现蓝色荧光，一旦与 Tb-DPA 整合形成 Ce-GMP-DPA@Tb-DPA 复合物，其荧光就会被抑制。由于能量从 Ce 转移到 Tb，所形成的 Ce-GMP-DPA@Tb-DPA 表现出 Tb-DPA 的绿色荧光。水的存在可以分解 Tb-DPA，从而阻止能量从 Ce 转移到 Tb，导致 Tb-DPA 的绿色发射减少，Ce-GMP-DPA 的蓝色发射恢复。因此，建立了一种比率荧光分析方法，用于在乙醇中进行从 0.2 到 90.0%的宽线性范围内的定量水检测。在乙醇中的检测限（LOD）低至 0.16%，在 THF 中的检测限为 0.62%，在乙腈中的检测限为 0.0076%。此外，设计了一个智能手机，它安装了 Color Picker APP 作为信号读取器和分析器，并与检测分析集成。以白酒为实际样品，该方法可用于现场高精度检测水（RSD<2.81%）。该开发平台具有成本低、携带方便、操作简单等优点，在实际应用中具有便携水检测的巨大潜力。
