State Key Laboratory of Natural Medicines, Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Basic Medical Sciences and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.
Nano Lett. 2021 Jun 9;21(11):4606-4614. doi: 10.1021/acs.nanolett.1c00548. Epub 2021 May 20.
The sensing and visualized monitoring of hydrogen sulfide (HS) in vivo is crucial to understand its physiological and pathological roles in human health and diseases. Common methods for HS detection require the destruction of the biosamples and are not suitable to be applied in vivo. In this Communication, we report a "turn-on" second near-infrared (NIR-II) luminescent approach for sensitive, real-time, and in situ HS detection, which is based on the absorption competition between the HS-responsive chromophores (compound ) and the NIR-II luminescent lanthanide nanoparticles. Specifically, the luminescence was suppressed by compound due to the competitive absorption of the incident light. In the presence of HS, the compound was bleached to recover the luminescence. Thanks to the deep tissue penetration depth and the low absorbance/scattering on biological samples of the NIR-II nanoprobes, the monitoring of the endogenous HS in lipopolysaccharide-induced liver inflammation was achieved, which is unattainable by the conventional histopathological and serological approaches.
体内硫化氢 (HS) 的感应和可视化监测对于理解其在人类健康和疾病中的生理和病理作用至关重要。常见的 HS 检测方法需要破坏生物样本，因此不适合在体内应用。在本通讯中，我们报告了一种“开启”的近红外二区 (NIR-II) 发光方法，用于灵敏、实时和原位 HS 检测，该方法基于 HS 响应发色团（化合物）与 NIR-II 发光镧系纳米粒子之间的吸收竞争。具体而言，由于入射光的竞争吸收，化合物导致发光被抑制。在存在 HS 的情况下，化合物被漂白以恢复发光。由于 NIR-II 纳米探针在生物样本中的深组织穿透深度和低吸收/散射，实现了内源性 HS 在脂多糖诱导的肝炎症中的监测，这是传统组织病理学和血清学方法无法实现的。
