Department of Pathology, Charles-Le Moyne Hospital, CISSS Montérégie-Centre, University of Sherbrooke, Longueuil, Canada.
Department of Pathology, McGill University, Montreal, Canada.
Cancer Cytopathol. 2021 Oct;129(10):759-771. doi: 10.1002/cncy.22442. Epub 2021 May 20.
At the time of intraoperative consultation, cytologic preparations including smears and imprints can be used in combination with frozen sections to increase diagnostic yield; however, these simple and rapid techniques are not adopted by all pathologists and their use varies considerably between institutions. In patients under investigation for suspected lymphoma, optimal triaging of tissue received fresh in pathology for lymphoma workup is paramount to maximize the odds of obtaining an accurate and clinically meaningful diagnosis and to avoid the need for additional procedures and delays in management, particularly in the current context in which core biopsies have become common practice as a first attempt to attain this goal. Imprint cytology is invaluable in this regard, also as these patients may not have a lymphoma but rather one of its clinical mimics. Herein, imprint cytology is used to approach fresh specimens received intraoperatively for lymphoma workup. More specifically, how these specimens are triaged for ancillary studies, such as flow cytometry, florescence in situ hybridization, or molecular analyses based on an interpretation of the touch imprints, is described. Detailed imprint cytological findings of typical benign and malignant lymphoid and nonlymphoid lesions are discussed and illustrated.
在术中咨询时，细胞学标本（包括涂片和印片）可与冷冻切片联合使用，以提高诊断率；然而，并非所有病理学家都采用这些简单快速的技术，其使用在不同机构之间差异很大。在疑似淋巴瘤患者的检查中，对新鲜病理组织进行优化分类，以进行淋巴瘤检查，对于最大限度提高获得准确且具有临床意义的诊断的几率至关重要，并避免需要额外的程序和延迟管理，尤其是在当前核心活检已成为实现这一目标的首次尝试的常见做法的情况下。印片细胞学在这方面非常有价值，因为这些患者可能没有淋巴瘤，而是具有其临床类似物。在此，使用印片细胞学方法来处理用于淋巴瘤检查的术中新鲜标本。更具体地说，描述了如何根据触摸印片的解释，对这些标本进行辅助研究（例如流式细胞术、荧光原位杂交或分子分析）的分类。讨论并说明了典型的良性和恶性淋巴样和非淋巴样病变的详细印片细胞学发现。
