Key Laboratory of Healthy Mariculture for the East China Sea, Fisheries College, Jimei University, Ministry of Agriculture, Xiamen, China.
State Key Laboratory of Large Yellow Croaker Breeding, Ningde, China.
Front Immunol. 2019 May 3;10:984. doi: 10.3389/fimmu.2019.00984. eCollection 2019.
is a temperature-dependent opportunistic pathogen which is associated with a variety of diseases in fish. During the development of "white nodules" disease, the expression of in was found to be significantly up-regulated at its virulent temperature of 18°C. The infection of -RNAi strain resulted in the onset time delay, reduction in mortality and infection symptoms in spleen of , and affected the bacterial tissue colonization. In order to reveal the effect of silencing of on the virulence regulation in and immune response in , dual RNA-seq was performed and a pathogen-host integration network was constructed. Our results showed that infection induced the expression of host genes related to immune response, but attenuated the expression of bacterial virulence genes. Novel integration was found between host immune genes and bacterial virulence genes, while , and played key roles in the network. Further analysis with GeneMANIA indicated that and might play key roles during the -dependent virulence regulation, which was in accordance with the reduced biofilm production, motility and virulence in -RNAi strain. Meanwhile, and were found to play key roles during the defense against , while , and were important targets for attacking to the host.
是一种温度依赖型机会致病菌，与鱼类的多种疾病有关。在“白色结节”病的发展过程中，发现其在毒力温度 18°C 时的表达显著上调。-RNAi 菌株的感染导致发病时间延迟、死亡率降低和脾脏感染症状减轻，并影响细菌组织定植。为了揭示沉默对毒力调节和免疫反应的影响，进行了双 RNA-seq 并构建了病原体-宿主整合网络。我们的结果表明，感染诱导了与免疫反应相关的宿主基因的表达，但减弱了细菌毒力基因的表达。在宿主免疫基因和细菌毒力基因之间发现了新的整合，而、和在网络中起关键作用。进一步的 GeneMANIA 分析表明和可能在依赖的毒力调节中发挥关键作用，这与 -RNAi 菌株中生物膜产生、运动性和毒力降低一致。同时，和在抵御中发挥关键作用，而、和是针对宿主的重要靶点。
