Zhang Baojian, Li Biao, Sun Chao, Tu Tao, Xiao Yichao, Liu Qiming
Department of Cardiology/Cardiac Catheterization Lab, Second Xiangya Hospital, Central South University Changsha, China.
Cardiac Care Unit, Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University Urumqi, Xinjiang Uygur Autonomous Region, China.
Am J Transl Res. 2021 Apr 15;13(4):3890-3905. eCollection 2021.
Acute myocardial infarction (AMI) seriously threatens human life. In this study we aimed to systemically analyze the function of key gene modules in human platelets in AMI. We used weighted gene co-expression network analysis (WGCNA) to construct a co-expression module, and analyzed the relationship between potential modules and clinical characteristics based on platelet RNA-seq RPKM count reads of 16 ST-segment elevation myocardial infarction (STEMI) patients and 16 non-STEMI (NSTEMI) patients provided by the GEO database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed with the DAVID tool. Hub genes were calculated by the Cytohubba package. A total of 3653 genes was selected to construct the co-expression modules. A significant correlation between BMI and the module with color of sky-blue in STEMI. In NSTEMI, there was a significant correlation between the sky blue module and CAD, the Salmon module and HT, and the Cyan module and HT. In STEMI, the Hub genes were mainly enriched in functions related to cell membrane signal transduction including and . In NSTEMI, the Hub genes are related mainly to energy metabolism in the sky-blue module including and ; they are mainly related to extracellular space and calcium binding in the Cyan module, including and . The hub genes in the Salmon module include and . Our results provide a framework for co-expression gene modules in STEMI and NSTEMI patients, and identify key targets as biomarkers for patients with different subtypes of AMI.
急性心肌梗死（AMI）严重威胁人类生命。在本研究中，我们旨在系统分析AMI患者人类血小板中关键基因模块的功能。我们使用加权基因共表达网络分析（WGCNA）构建共表达模块，并基于GEO数据库提供的16例ST段抬高型心肌梗死（STEMI）患者和16例非STEMI（NSTEMI）患者的血小板RNA-seq RPKM计数读数，分析潜在模块与临床特征之间的关系。使用DAVID工具进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。通过Cytohubba软件包计算枢纽基因。共选择3653个基因构建共表达模块。在STEMI中，BMI与天蓝色模块之间存在显著相关性。在NSTEMI中，天蓝色模块与CAD、鲑鱼色模块与HT以及青色模块与HT之间存在显著相关性。在STEMI中，枢纽基因主要富集于与细胞膜信号转导相关的功能，包括[具体功能1]和[具体功能2]。在NSTEMI中，天蓝色模块中的枢纽基因主要与能量代谢相关，包括[具体基因1]和[具体基因2]；青色模块中的枢纽基因主要与细胞外空间和钙结合相关，包括[具体基因3]和[具体基因4]。鲑鱼色模块中的枢纽基因包括[具体基因5]和[具体基因6]。我们的结果为STEMI和NSTEMI患者的共表达基因模块提供了一个框架，并确定了关键靶点作为不同亚型AMI患者的生物标志物。
