John Gordon, Schwarz Frank, Kravchenko Alexandra, Ommerborn Michelle Alicia, Becker Jürgen
Department of Oral Surgery and Central Admittance, Westdeutsche Kieferklinik, Heinrich Heine University Düsseldorf, Moorenstr. 5, D-40225, Düsseldorf, Germany.
Department of Oral Surgery and Implantology, Carolinum, Goethe University, Frankfurt, Germany.
Int J Implant Dent. 2021 May 21;7(1):51. doi: 10.1186/s40729-021-00326-x.
The aim of the current study was the evaluation of initial biofilm adhesion and development on laser-microtextured implant collar surfaces and the examination of effectivity of different biofilm management methods.
Initial biofilm formation was investigated on hydrophobic machined and laser-microtextured (Laser-Lok) titanium surfaces and hydrophobic machined and laser-microtextured (Laser-Lok) titanium aluminium vanadium surfaces and compared to hydrophobic smooth pickled titanium surfaces, hydrophilic smooth and acid etched titanium surfaces, hydrophobic sandblasted large grid and acid etched titanium surfaces (titanium Promote) via erythrosine staining and subsequent histomorphometrical analysis and scanning electron microscopic investigations. After decontamination procedures, performed via tooth brushing and glycine powder blasting, clean implant surface was detected via histomorphometrical analysis.
After 24 h mean initial plaque area was detected in the following descending order: smooth pickled titanium > titanium Promote > hydrophilic smooth and acid etched titanium > Laser-Lok titanium > Laser-Lok titanium aluminium vanadium. The same order was determined after 48 h of biofilm formation. After glycine powder blasting all samples depicted almost 100% clean implant surface. After tooth brushing, Laser-Lok titanium (67.19%) and Laser-Lok titanium aluminium vanadium (69.80%) showed significantly more clean implant surface than the other structured surfaces, hydrophilic smooth and acid etched titanium (50.34%) and titanium Promote (33.89%). Smooth pickled titanium showed almost complete clean implant surface (98.84%) after tooth brushing.
Both Laser-Lok surfaces showed less initial biofilm formation after 24 and 48 h than the other implant surfaces. In combination with the significant higher clean implant surfaces after domestic decontamination procedure via tooth brushing, both Laser-Lok surfaces could be a candidate for modified implant and abutment designs, especially in transmucosal areas.
本研究的目的是评估激光微纹理种植体颈部表面的初始生物膜黏附与形成情况，并检验不同生物膜管理方法的有效性。
通过赤藓红染色、随后的组织形态计量学分析和扫描电子显微镜研究，对疏水性机械加工和激光微纹理（Laser-Lok）钛表面、疏水性机械加工和激光微纹理（Laser-Lok）钛铝钒表面的初始生物膜形成情况进行研究，并与疏水性光滑酸洗钛表面、亲水性光滑和酸蚀钛表面、疏水性喷砂大网格和酸蚀钛表面（钛促进型）进行比较。在通过刷牙和甘氨酸粉末喷砂进行去污程序后，通过组织形态计量学分析检测清洁的种植体表面。
24小时后，平均初始菌斑面积按以下降序排列：光滑酸洗钛＞钛促进型＞亲水性光滑和酸蚀钛＞Laser-Lok钛＞Laser-Lok钛铝钒。生物膜形成48小时后确定了相同的顺序。甘氨酸粉末喷砂后，所有样本的种植体表面几乎100%清洁。刷牙后，Laser-Lok钛（67.19%）和Laser-Lok钛铝钒（69.80%）的种植体表面清洁度明显高于其他结构化表面，即亲水性光滑和酸蚀钛（50.34%）和钛促进型（33.89%）。光滑酸洗钛刷牙后种植体表面几乎完全清洁（98.84%）。
与其他种植体表面相比，两种Laser-Lok表面在24小时和48小时后的初始生物膜形成较少。结合通过刷牙进行家庭去污程序后显著更高的种植体表面清洁度，两种Laser-Lok表面都可能是改良种植体和基台设计的候选材料，特别是在穿黏膜区域。
