Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
Food Funct. 2021 Jul 5;12(13):5862-5875. doi: 10.1039/d1fo00282a.
The aim of this study was to investigate the effects of Smilax china L. flavonoid (SCF) on obesity and changes in gut microbiota high-fat/high-sucrose (HFHS)-fed mice. Male C57BL/6 mice fed either a normal-chow (NC) or a HFHS diet were treated with SCF for 12 weeks. The effect of SCF on the composition of gut microbiota was assessed by 16S rDNA sequencing. SCFA levels in the caecum were quantified by GC-MS. SCF supplementation alleviated the body weight gain, fat accumulation, serum lipid parameters, and hepatic steatosis and improved glucose homeostasis. SCF significantly increased plasma adiponectin level, adiponectin-receptor-gene (AdipoR1 and AdipoR2) expression in the liver, activated AMPKα, downregulated the expression of SREBP1-c, FAS, and ACCα, and upregulated the expression of PPARα, CPT-1α, and UCP-1. The anti-obesity effects of SCF might be through upregulation of adiponectin-receptor/AMPK signalling to improve lipid metabolism. SCF reversed HFHS-induced dysbiosis of gut microbiota and decreased SCFA production in the caecum, thus reducing energy absorption and leading to loss of body weight. Spearman's correlation analysis revealed significant correlations between obesity phenotypes, SCFA levels, and changes in gut microbiota. The results showed that SCF may be an effective dietary supplement that is useful for suppressing the development of obesity and associated disorders.
本研究旨在探讨菝葜黄酮（SCF）对肥胖和高脂肪/高蔗糖（HFHS）喂养小鼠肠道微生物群变化的影响。雄性 C57BL/6 小鼠分别用正常饲料（NC）或 HFHS 饲料喂养，并给予 SCF 治疗 12 周。通过 16S rDNA 测序评估 SCF 对肠道微生物群组成的影响。通过 GC-MS 定量测定盲肠中 SCFA 的水平。SCF 补充缓解了体重增加、脂肪堆积、血清脂质参数、肝脂肪变性和改善葡萄糖稳态。SCF 显著增加了血浆脂联素水平、肝脏中脂联素受体基因（AdipoR1 和 AdipoR2）的表达，激活了 AMPKα，下调了 SREBP1-c、FAS 和 ACCα 的表达，上调了 PPARα、CPT-1α 和 UCP-1 的表达。SCF 的抗肥胖作用可能是通过上调脂联素受体/AMPK 信号来改善脂质代谢。SCF 逆转了 HFHS 诱导的肠道微生物群失调和盲肠中 SCFA 产量的降低，从而减少了能量吸收，导致体重减轻。Spearman 相关性分析显示肥胖表型、SCFA 水平和肠道微生物群变化之间存在显著相关性。结果表明，SCF 可能是一种有效的膳食补充剂，有助于抑制肥胖及其相关疾病的发展。
