Pan Lixia, Ye Hangyu, Pi Xionge, Liu Wei, Wang Zhao, Zhang Yinjun, Zheng Jianyong
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Microbiol. 2023 Feb 2;14:1092729. doi: 10.3389/fmicb.2023.1092729. eCollection 2023.
Flavonoids have antiviral, antitumor, anti-inflammatory, and other biological activities. They have high market value and are widely used in food and medicine fields. They also can regulate gut microbiota and promote human health. However, only a few flavonoids have been reported for their regulatory effects on human gut microbiota.
The effects of hesperidin, hesperetin-7-O-glucoside, hesperetin, naringin, prunin, naringenin, rutin, isoquercitrin, and quercetin on gut microbiota structural and metabolic differences in healthy subjects were studied by means of simulated fermentation technology.
Results showed that the nine kinds of flavonoids mentioned above, especially hesperetin-7-O-glucoside, prunin, and isoquercitrin, were found to have more effect on the structure of human gut microbiota, and they could significantly enhance ( < 0.05). After 24 h of simulated fermentation, the relative abundance of intestinal probiotics (e.g., ) was increased by the three flavonoids and rutin. Furthermore, the relative abundance of potential pathogenic bacteria was decreased by the addition of hesperetin-7-O-glucoside, naringin, prunin, rutin, and isoquercitrin (e.g., and ). Notably, prunin could also markedly decrease the content of HS, NH, and short-chain fatty acids. This performance fully demonstrated its broad-spectrum antibacterial activity.
This study demonstrates that flavonoids can regulate the imbalance of gut microbiota, and some differences in the regulatory effect are observed due to different structures. This work provides a theoretical basis for the wide application of flavonoids for food and medicine.
黄酮类化合物具有抗病毒、抗肿瘤、抗炎等生物活性。它们具有很高的市场价值，广泛应用于食品和医药领域。它们还可以调节肠道微生物群，促进人类健康。然而，只有少数黄酮类化合物对人类肠道微生物群的调节作用有报道。
采用模拟发酵技术研究橙皮苷、橙皮素-7-O-葡萄糖苷、橙皮素、柚皮苷、樱草糖苷、柚皮素、芦丁、异槲皮苷和槲皮素对健康受试者肠道微生物群结构和代谢差异的影响。
结果表明，上述九种黄酮类化合物，尤其是橙皮素-7-O-葡萄糖苷、樱草糖苷和异槲皮苷，对人类肠道微生物群的结构有更大影响，且能显著增强（<0.05）。模拟发酵24小时后，这三种黄酮类化合物和芦丁可使肠道益生菌（如）的相对丰度增加。此外，添加橙皮素-7-O-葡萄糖苷、柚皮苷、樱草糖苷、芦丁和异槲皮苷可降低潜在病原菌的相对丰度（如和）。值得注意的是，樱草糖苷还可显著降低硫化氢、氨和短链脂肪酸的含量。这一表现充分证明了其广谱抗菌活性。
本研究表明黄酮类化合物可调节肠道微生物群的失衡，且由于结构不同观察到调节效果存在一些差异。这项工作为黄酮类化合物在食品和医药中的广泛应用提供了理论依据。
