Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 10607, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei, 10617, Taiwan.
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 10607, Taiwan; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 10617, Taiwan.
Anal Chim Acta. 2023 Jan 25;1239:340651. doi: 10.1016/j.aca.2022.340651. Epub 2022 Nov 21.
Epidemiological control and public health monitoring during the outbreaks of infectious viral diseases rely on the ability to detect viral pathogens. Here we demonstrate a rapid, sensitive, and selective nanotechnology-enhanced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection based on the surface-enhanced Raman scattering (SERS) responses from the plasma-engineered, variant-specific antibody-functionalized silver microplasma-engineered nanoassemblies (AgMEN) interacting with the SARS-CoV-2 spike (S) and nucleocapsid (N) proteins. The three-dimensional (3D) porous AgMEN with plasmonic-active nanostructures provide a high sensitivity to virus detection via the remarkable SERS signal collection. Moreover, the variant-specific antibody-functionalization on the SERS-active AgMEN enabled the high selectivity of the SARS-CoV-2 S variants, including wild-type, Alpha, Delta, and Omicron, under the simulated human saliva conditions. The exceptional ultrahigh sensitivity of our SERS biosensor was demonstrated via SARS-CoV-2 S and N proteins at the detection limit of 1 fg mL and 0.1 pg mL, respectively. Our work demonstrates a versatile SERS-based detection platform can be applied for the ultrasensitive detection of virus variants, infectious diseases, and cancer biomarkers.
在传染病爆发期间，流行病学控制和公共卫生监测依赖于检测病毒病原体的能力。在这里，我们展示了一种基于等离子体工程化、具有变体特异性的抗体功能化银微等离子体工程纳米组装体（AgMEN）与 SARS-CoV-2 刺突（S）和核衣壳（N）蛋白相互作用的表面增强拉曼散射（SERS）响应的快速、灵敏和选择性 SARS-CoV-2 检测的纳米技术增强方法。具有等离子体活性纳米结构的三维（3D）多孔 AgMEN 通过显著的 SERS 信号收集提供了对病毒检测的高灵敏度。此外，SERS 活性 AgMEN 上的变体特异性抗体功能化使得在模拟人类唾液条件下对 SARS-CoV-2 S 变体（包括野生型、Alpha、Delta 和 Omicron）具有高选择性。我们的 SERS 生物传感器通过 SARS-CoV-2 S 和 N 蛋白的检测限分别达到 1 fg mL 和 0.1 pg mL，证明了其卓越的超高灵敏度。我们的工作展示了一种多功能的基于 SERS 的检测平台，可用于超灵敏检测病毒变体、传染病和癌症生物标志物。
