Center on Membrane Protein Production and Analysis (COMPPÅ), New York Structural Biology Center (NYSBC), New York, NY 10027, USA.
Rockefeller University, New York, NY 10065, USA.
J Mol Biol. 2021 Jul 23;433(15):167055. doi: 10.1016/j.jmb.2021.167055. Epub 2021 May 20.
We describe an enhancement of traditional genomics-based approaches to improve the success of structure determination of membrane proteins. Following a broad screen of sequence space to identify initial expression-positive targets, we employ a second step to select orthologs with closely related sequences to these hits. We demonstrate that a greater percentage of these latter targets express well and are stable in detergent, increasing the likelihood of identifying candidates that will ultimately yield structural information.
我们描述了一种改进的传统基于基因组学的方法，以提高膜蛋白结构测定的成功率。在广泛筛选序列空间以识别初始表达阳性的靶标后，我们采用第二步来选择与这些命中靶标具有密切相关序列的同源物。我们证明，这些后一类靶标中有更大比例的表达良好且在去污剂中稳定，这增加了最终获得结构信息的候选物的可能性。
