Institute of Translational Research, Department of Molecular Biology, Asian Healthcare Foundation, AIG Hospitals Complex, Mindspace Road, Gachibowli, Hyderabad, 500032, Telangana, India.
Institute of Translational Research, Department of Molecular Biology, Asian Healthcare Foundation, AIG Hospitals Complex, Mindspace Road, Gachibowli, Hyderabad, 500032, Telangana, India.
Int J Infect Dis. 2021 Jul;108:183-186. doi: 10.1016/j.ijid.2021.05.034. Epub 2021 May 19.
The efficacy of COVID-19 vaccines to generate immunological memory post-vaccination has not previously been studied.
To assess immunological memory in previously SARS-CoV-2 infected individuals after a single dose of mRNA vaccine.
Healthcare workers (n = 280) were enrolled after obtaining written informed consent and grouped under previously infected and no prior exposure (reverse transcription-polymerase chain reaction positive and negative, respectively). Blood was drawn at baseline and post-vaccination (single dose of COVISHIELD) for enumerating neutralizing antibodies by chemiluminescence and memory T- and B-cells by flow cytometry.
Post vaccination, compared with the no prior exposure group, the previously infected group had higher levels of: antibody response (1124.73 ± 869.13 vs 94.23 ± 140.06 AU/ml, p = 0.0001); CD4 memory T-cells: central memory CCR7+CD45RA- (p = 0.0001), effector memory CCR7-/CD45RA- (p = 0.01); total CD8+ T-cells (p = 0.004); CD8+ naïve T-cells CCR7+CD45RA+ (p = 0.01); and memory B-cells CD20+CD27+ (p = 0.0001).
Single-dose vaccination elicited higher neutralizing antibody response and protective immunity in individuals who had recovered from SARS-CoV-2 infection compared with those with no prior exposure.
COVID-19 疫苗接种后产生免疫记忆的效果此前尚未研究过。
评估单剂 mRNA 疫苗接种后既往 SARS-CoV-2 感染者的免疫记忆。
在获得书面知情同意后，招募了 280 名医护人员，并根据既往感染和无既往暴露（分别为逆转录-聚合酶链反应阳性和阴性）将其分为两组。在基线和接种疫苗后（单次接种 COVISHIELD）抽取血液，通过化学发光法检测中和抗体，通过流式细胞术检测记忆 T 细胞和 B 细胞。
与无既往暴露组相比，既往感染组在接种疫苗后具有更高水平的：抗体反应（1124.73±869.13 与 94.23±140.06 AU/ml，p=0.0001）；CD4 记忆 T 细胞：中央记忆 CCR7+CD45RA-（p=0.0001），效应记忆 CCR7-/CD45RA-（p=0.01）；总 CD8+T 细胞（p=0.004）；CD8+幼稚 T 细胞 CCR7+CD45RA+（p=0.01）；以及记忆 B 细胞 CD20+CD27+（p=0.0001）。
与无既往暴露者相比，既往 SARS-CoV-2 感染者单剂疫苗接种可引起更高的中和抗体反应和保护性免疫。
