State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, 610041, China.
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, 610041, China.
Microb Pathog. 2021 Aug;157:104957. doi: 10.1016/j.micpath.2021.104957. Epub 2021 May 20.
The present study aimed to assess the impact of sodium new houttuyfonate (SNH) on growth and biofilm formation of Streptococcus mutans, and the combinatorial effects of SNH with cariostatic agents. The effects of SNH on S. mutans planktonic cultures were assessed by growth curve assay. The effects of SNH on S. mutans biofilm and extracellular polysaccharides (EPS) production were observed via crystal violet (CV) assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, colony-forming unit (CFU) counting assay, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Quantitative real-time polymerase chain reaction (qPCR) was applied to investigate the regulatory effects of SNH on the expression of virulence genes of S. mutans. Checkerboard microdilution assay was performed to investigate the combinatorial effects of SNH with two common cariostatic agents. SNH acted as an inhibitor on planktonic cell growth, biofilm formation and EPS production of S. mutans. SNH also downregulated the expression of gtfBCD and comDE systems and exhibited synergism with chlorhexidine (CHX). In conclusion, this study indicated a possibility for SNH to become an anticaries agents by its antimicrobial activity and synergistic effects with CHX against S. mutans.
本研究旨在评估钠牛磺酸（SNH）对变形链球菌生长和生物膜形成的影响，以及 SNH 与防龋剂联合使用的协同作用。通过生长曲线测定法评估 SNH 对变形链球菌浮游培养物的影响。通过结晶紫（CV）测定法、3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化盐（MTT）测定法、菌落形成单位（CFU）计数测定法、扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）观察 SNH 对变形链球菌生物膜和细胞外多糖（EPS）产生的影响。应用实时定量聚合酶链反应（qPCR）研究 SNH 对变形链球菌毒力基因表达的调控作用。棋盘微量稀释法研究 SNH 与两种常见防龋剂的联合作用。SNH 对变形链球菌浮游细胞生长、生物膜形成和 EPS 产生均具有抑制作用。SNH 还下调了 gtfBCD 和 comDE 系统的表达，并与洗必泰（CHX）表现出协同作用。综上所述，本研究表明 SNH 具有抗微生物活性，并与 CHX 对变形链球菌表现出协同作用，有可能成为一种抗龋剂。
