Laboratório de Pesquisa Em Bioquímica e Biologia Molecular de Micro-organismos (LaPeBBioM), Grupo de Pesquisa Em Bioquímica e Biologia Molecular de Micro-organismos, Centro de Ciências Químicas, Farmacêuticas e de Alimentos (CCQFA), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil.
Curso de Farmácia, Centro de Ciências Químicas, Farmacêuticas e de Alimentos (CCQFA), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil.
Microb Pathog. 2021 Sep;158:104975. doi: 10.1016/j.micpath.2021.104975. Epub 2021 May 19.
In early December 2019, an outbreak of coronavirus disease 2019 caused by a new strain of coronavirus (SARS-CoV-2), occurred in the city of Wuhan, Hubei Province, China. On January 30, 2020, the World Health Organization (WHO) declared the outbreak a public health emergency of international concern. Since then, frontline healthcare professionals have been experiencing extremely stressful situations and damage to their physical and mental health. These adverse conditions cause stress and biochemical, hematological, and inflammatory changes, as well as oxidative damage, and could be potentially detrimental to the health of the individual. The study population consisted of frontline health professionals working in BHU in a city in southern Brazil. Among the 45 participants, two were infected with the SARS-CoV-2 virus and were diagnosed using immunochromatographic tests such as salivary RT-LAMP and qRT-PCR. We also evaluated biochemical, hematological, inflammatory, and oxidative stress markers in the participants. The infected professionals (CoV-2-Prof) showed a significant increase in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol, lactic dehydrogenase, lymphocytes, and monocytes. In this group, the levels of uric acid, triglycerides, leukocytes, neutrophils, hemoglobin, hematocrit, and platelets decreased. In the group of uninfected professionals (NoCoV-2-Prof), significant increase in HDL levels and the percentages of eosinophils and monocytes, was observed. Further, in this group, uric acid, LDH, triglyceride, and cholesterol levels, and the hematocrit count and mean corpuscular volume were significantly reduced. Both groups showed significant inflammatory activity with changes in the levels of C-reactive protein and mucoprotein. The NoCoV-2-Prof group showed significantly elevated plasma cortisol levels. To our kowledge, this study is the first to report the use of the RT-LAMP method with the saliva samples of health professionals, to evalute of SARS-CoV-2.
2019 年 12 月初，一种新型冠状病毒（SARS-CoV-2）引发的 2019 年冠状病毒病（COVID-19）在湖北省武汉市爆发。2020 年 1 月 30 日，世界卫生组织（WHO）宣布该疫情为国际关注的突发公共卫生事件。自那时以来，一线医护人员一直面临着极其紧张的情况和身心健康的损害。这些不利条件会导致压力以及生化、血液学和炎症变化，以及氧化损伤，并且可能对个体健康造成潜在危害。研究人群由在巴西南部城市 BHU 工作的一线卫生专业人员组成。在 45 名参与者中，有 2 人感染了 SARS-CoV-2 病毒，并通过唾液 RT-LAMP 和 qRT-PCR 等免疫层析试验进行了诊断。我们还评估了参与者的生化、血液学、炎症和氧化应激标志物。感染的专业人员（CoV-2-Prof）的丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、胆固醇、乳酸脱氢酶、淋巴细胞和单核细胞水平显著升高。在该组中，尿酸、甘油三酯、白细胞、中性粒细胞、血红蛋白、血细胞比容和血小板水平降低。在未感染的专业人员（NoCoV-2-Prof）组中，观察到高密度脂蛋白（HDL）水平以及嗜酸性粒细胞和单核细胞的百分比显著增加。此外，在该组中，尿酸、LDH、甘油三酯和胆固醇水平以及血细胞比容计数和平均红细胞体积显著降低。两组均表现出明显的炎症活性，C-反应蛋白和粘蛋白水平发生变化。NoCoV-2-Prof 组的血浆皮质醇水平显著升高。据我们所知，这项研究首次报告了使用 RT-LAMP 法结合唾液样本来评估 SARS-CoV-2 的情况。
