Department of Chemical, Pharmaceutical, and Food Sciences, Microorganism Biochemistry and Molecular Biology Research Laboratory, (LAPEBBIOM), Federal University of Pelotas, Pelotas 96010-610, RS, Brazil.
Department of Chemical, Pharmaceutical, and Food Sciences, Laboratory for Lipidomic and Bio-Organic Research, Bioforensic Research Group, Federal University of Pelotas, Pelotas 96010-610, RS, Brazil.
Biosensors (Basel). 2023 Mar 13;13(3):378. doi: 10.3390/bios13030378.
The COVID-19 pandemic has caused an unprecedented health and economic crisis, highlighting the importance of developing new molecular tools to monitor and detect SARS-CoV-2. Hence, this study proposed to employ the carrageenan extracted from algae as a probe for SARS-CoV-2 virus binding capacity and potential use in molecular methods. specimens were collected in the Chilean subantarctic ecoregion, and the carrageenan was extracted -using a modified version of Webber's method-, characterized, and quantified. After 24 h of incubation with an inactivated viral suspension, the carrageenan's capacity to bind SARS-CoV-2 was tested. The probe-bound viral RNA was quantified using the reverse transcription and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods. Our findings showed that carrageenan extraction from seaweed has a similar spectrum to commercial carrageenan, achieving an excellent proportion of binding to SARS-CoV-2, with a yield of 8.3%. Viral RNA was also detected in the RT-LAMP assay. This study shows, for the first time, the binding capacity of carrageenan extracted from , which proved to be a low-cost and highly efficient method of binding to SARS-CoV-2 viral particles.
新冠疫情引发了前所未有的健康和经济危机，凸显了开发新的分子工具来监测和检测 SARS-CoV-2 的重要性。因此，本研究拟利用从藻类中提取的卡拉胶作为 SARS-CoV-2 病毒结合能力的探针，并探讨其在分子方法中的潜在用途。本研究采集了智利亚南极生态区的样本，使用改良的 Webber 法提取、表征和定量卡拉胶。在与灭活病毒悬液孵育 24 小时后，检测卡拉胶结合 SARS-CoV-2 的能力。使用逆转录和逆转录环介导等温扩增（RT-LAMP）方法定量探针结合的病毒 RNA。我们的研究结果表明，从海藻中提取的卡拉胶与商业卡拉胶具有相似的光谱，对 SARS-CoV-2 的结合效果极佳，结合率达到 8.3%。在 RT-LAMP 检测中也检测到了病毒 RNA。本研究首次证明了从海藻中提取的卡拉胶的结合能力，这是一种低成本、高效的结合 SARS-CoV-2 病毒颗粒的方法。
