School of Biological Science and Engineering Shaanxi Key Laboratory of Bioresources, Shaanxi University of Technology, Hanzhong, China.
School of Biological Science and Engineering Shaanxi Key Laboratory of Bioresources, Shaanxi University of Technology, Hanzhong, China.
Food Chem. 2021 Nov 1;361:130061. doi: 10.1016/j.foodchem.2021.130061. Epub 2021 May 11.
Two collagens were made from giant salamander (Andrias davidianus) skin by using acid and pepsin extraction methods. The yields of acid-soluble and pepsin-soluble collagens were 26.9 and 58.7%, respectively. The results of spectrum, electrophoresis and amino acid analysis showed that they were type 1 collagen with two α and one β peptides and high imino acid content. They had low solubility at a pH above 6 or salt concentration over 5%. The pepsin-soluble collagen had a better emulsion activity index. The odorants in raw skin and collagens were identified and evaluated using gas-chromatography mass-spectrometer and olfactometry methods and sensory analysis. The fishy and fatty off-odors in skin were not perceivable in the collagens. Sour, ammonia-like, and acrid off-odors were found in the collagens due to acid and enzymatic hydrolysis and protein degradation. The off-odor intensity of pepsin-soluble collagen was low. It could be considered a good and safe collagen material.
两种胶原蛋白是从大鲵（Andrias davidianus）皮中通过酸提取和胃蛋白酶提取方法得到的。酸溶性胶原蛋白和胃蛋白酶溶性胶原蛋白的产率分别为 26.9%和 58.7%。光谱、电泳和氨基酸分析的结果表明，它们是具有两个α和一个β肽和高亚氨基酸含量的 1 型胶原蛋白。它们在 pH 值高于 6 或盐浓度超过 5%时的溶解度较低。胃蛋白酶溶性胶原蛋白具有更好的乳化活性指数。使用气相色谱-质谱联用仪和嗅闻仪法以及感官分析对生皮和胶原蛋白中的气味物质进行了鉴定和评价。鱼腥味和脂肪味在胶原蛋白中不可察觉。由于酸和酶解以及蛋白质降解，在胶原蛋白中发现了酸味、氨味和刺鼻味等不良气味。胃蛋白酶溶性胶原蛋白的不良气味强度较低。它可以被认为是一种良好且安全的胶原蛋白材料。
