Department of Biology, College of Arts and Sciences, Indiana University, Bloomington, IN, USA.
Department of Microbiology and Immunology and Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases, University of Louisville School of Medicine, Louisville, KY, USA.
Virology. 2021 Aug;560:34-42. doi: 10.1016/j.virol.2021.04.009. Epub 2021 May 6.
Alphaviruses are positive sense, RNA viruses commonly transmitted by an arthropod vector to a mammalian or avian host. In recent years, a number of the Alphavirus members have reemerged as public health concerns. Transmission from mosquito vector to vertebrate hosts requires an understanding of the interaction between the virus and both vertebrate and insect hosts to develop rational intervention strategies. The current study uncovers a novel role for capsid protein during Chikungunya virus replication whereby the interaction with viral RNA in the E1 coding region regulates protein synthesis processes early in infection. Studies done in both the mammalian and mosquito cells indicate that interactions between viral RNA and capsid protein have functional consequences that are host species specific. Our data support a vertebrate-specific role for capsid:vRNA interaction in temporally regulating viral translation in a manner dependent on the PI3K-AKT-mTOR pathway.
甲病毒是正链 RNA 病毒，通常通过节肢动物媒介传播给哺乳动物或禽类宿主。近年来，一些甲病毒成员重新成为公共卫生关注的焦点。从蚊子媒介到脊椎动物宿主的传播需要了解病毒与脊椎动物和昆虫宿主之间的相互作用，以制定合理的干预策略。本研究揭示了衣壳蛋白在基孔肯雅病毒复制过程中的一个新作用，即与 E1 编码区病毒 RNA 的相互作用调节感染早期的蛋白质合成过程。在哺乳动物和蚊子细胞中的研究表明，病毒 RNA 和衣壳蛋白之间的相互作用具有宿主种特异性的功能后果。我们的数据支持衣壳：vRNA 相互作用在脊椎动物中具有特定的作用，以依赖于 PI3K-AKT-mTOR 途径的方式在时间上调节病毒翻译。
