Rogers Ann E, Hu Yi-Juan, Yue Ye, Wissel Emily F, Petit Iii Robert A, Jarrett Simone, Christie Jennifer, Read Timothy D
Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, United States of America.
Department of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA, United States of America.
PeerJ. 2021 May 11;9:e11406. doi: 10.7717/peerj.11406. eCollection 2021.
There are about 15 million Americans working full-time on evening, night, or rotating shifts. Between 48% and 81.9% of those working rotating or night shifts report abdominal pain, constipation, diarrhea and other symptoms of functional bowel disorders. The basis for this high prevalence of functional bowel disorders, including irritable bowel syndrome (IBS), among shift workers is unknown. Animal studies, however, suggest that circadian disruption, similar to that in shift workers, may contribute to the development of GI complaints among shift workers by altering the composition and normal diurnal rhythmicity of the resident intestinal microbes. Therefore, the present study was designed to determine if there were differences in (1) composition and diversity of the microbiome of night shift workers compared to day shift workers; and (2) the composition and diversity of the microbiome among shift workers experiencing functional bowel symptoms compared to shift workers who did not experience functional bowel symptoms.
Fifty-one full time staff nurses who worked either 12-hour day or night shifts completed demographic information, and the Rome III IBS module. They also collected two samples of gut microbiota before the beginning and at the end of their last work shift on day 14, using validated field-tested methods consistent with the Human Microbiome Project. After DNA extraction, 16S rRNA sequencing and assignment to the genus level was completed, samples were then compared to determine if there were (1) differences in the diversity and profile of the microbiome by shift type; (2) if there were differences in the microbiome by time of day for collection; and (3) whether there were differences in the diversity and profile of the microbiome of nurses with IBS and those without IBS.
There were no differences in alpha or beta diversity of gut microbiota when specimens from day and night shift nurses were compared. There were however marginal differences in beta diversity when specimens collected at the beginning and end of the shifts were compared, with seven OTUs being differentially abundant when collected from day shift workers in the evening. There were also three OTUs to be differentially abundant in participants reporting IBS symptoms.
约有1500万美国人全职从事晚班、夜班或轮班工作。在从事轮班或夜班工作的人群中，48%至81.9%的人报告有腹痛、便秘、腹泻及其他功能性肠病症状。包括肠易激综合征（IBS）在内的功能性肠病在轮班工作者中如此高发的原因尚不清楚。然而，动物研究表明，与轮班工作者类似的昼夜节律紊乱，可能通过改变肠道常驻微生物的组成和正常昼夜节律，导致轮班工作者出现胃肠道不适。因此，本研究旨在确定：（1）与日班工作者相比，夜班工作者微生物组的组成和多样性是否存在差异；（2）有功能性肠病症状的轮班工作者与无功能性肠病症状的轮班工作者相比，其微生物组的组成和多样性是否存在差异。
51名全职护士，她们要么上12小时白班，要么上12小时夜班，完成了人口统计学信息及罗马III型肠易激综合征模块调查。她们还在第14天最后一个工作日开始前和结束时，使用与人类微生物组计划一致的经过验证的现场测试方法，采集了两份肠道微生物群样本。DNA提取后，完成16S rRNA测序并将其分类到属水平，然后对样本进行比较，以确定：（1）按轮班类型划分，微生物组的多样性和特征是否存在差异；（2）按采集时间划分，微生物组是否存在差异；（3）患有肠易激综合征的护士与未患肠易激综合征的护士，其微生物组的多样性和特征是否存在差异。
比较日班和夜班护士的样本时，肠道微生物群的α或β多样性没有差异。然而，比较轮班开始和结束时采集的样本时，β多样性存在微小差异，从日班工作者晚上采集的样本中有7个操作分类单元丰度不同。报告有肠易激综合征症状的参与者中也有3个操作分类单元丰度不同。
