Department of Pathology and Immunology, Texas Children's Microbiome Center.
Departments of Biostatistics and Office of Nursing Research.
J Clin Gastroenterol. 2020 Feb;54(2):175-183. doi: 10.1097/MCG.0000000000001107.
Altered microbial diversity has been associated with gastrointestinal (GI) symptoms in persons with irritable bowel syndrome (IBS). Less is known about the relationship of microbiome with extraintestinal pain and psychological distress symptoms and quality of life (QOL) in persons with IBS. We aimed to evaluate the relationship of fecal microbiota to GI symptoms, stool consistency, psychological distress, extraintestinal pain, and QOL in participants meeting Rome III criteria for IBS.
Seventy-six women completed a 28-day diary that included GI, stool consistency, psychological distress, and extraintestinal pain ratings. Participants completed the IBS-Specific Quality of Life questionnaire. Stool samples were collected and analyzed by 16S rRNA gene sequencing. Principal component analysis was performed and the first 2 components (PC1, PC2) were used to test relationships among bacterial families and clinical measures.
Participants were categorized as IBS constipation (n=22), IBS diarrhea (n=39), IBS mixed (n=13), and IBS unsubtyped (n=2). There was a significant group effect for the Firmicutes to Bacteroidetes ratio and PC1. Lower microbial diversity and richness were associated with increased urgency and extraintestinal pain, worse QOL, and looser stools. Lower extraintestinal pain was associated with increased Rikenellaceae, Christensenellaceae, Dehalobabacteriaceae, Oscillospiraceae, Mogibacteriaceae, Ruminococcaceae, Sutterellaceae, Desulfovibrionaceae, and Erysipelotrichaceae abundances. QOL was positively associated with many of these same bacterial families. Higher Firmicutes to Bacteroidetes ratio was positively associated with loose stools. There were no statistically significant relationships between daily psychological distress or abdominal pain and bacterial families.
Stool microbial diversity and composition are linked to daily extraintestinal symptoms, stool consistency, and QOL in women with IBS.
改变的微生物多样性与肠易激综合征（IBS）患者的胃肠道（GI）症状有关。关于微生物组与肠外疼痛和心理困扰症状以及 IBS 患者的生活质量（QOL）之间的关系知之甚少。我们旨在评估符合罗马 III 标准的 IBS 患者粪便微生物群与 GI 症状、粪便稠度、心理困扰、肠外疼痛和 QOL 的关系。
76 名女性完成了为期 28 天的日记，其中包括 GI、粪便稠度、心理困扰和肠外疼痛评分。参与者完成了 IBS 特异性生活质量问卷。收集粪便样本并通过 16S rRNA 基因测序进行分析。进行主成分分析，使用前 2 个成分（PC1、PC2）测试细菌家族与临床测量之间的关系。
参与者被分为 IBS 便秘（n=22）、IBS 腹泻（n=39）、IBS 混合（n=13）和 IBS 未分型（n=2）。Firmicutes 与 Bacteroidetes 比值和 PC1 存在显著的组间效应。微生物多样性和丰富度降低与紧迫性增加和肠外疼痛、QOL 恶化以及粪便变稀有关。较低的肠外疼痛与 Rikenellaceae、Christensenellaceae、Dehalobabacteriaceae、Oscillospiraceae、Mogibacteriaceae、Ruminococcaceae、Sutterellaceae、Desulfovibrionaceae 和 Erysipelotrichaceae 丰度增加有关。QOL 与许多相同的细菌家族呈正相关。较高的 Firmicutes 与 Bacteroidetes 比值与粪便变稀呈正相关。每日心理困扰或腹痛与细菌家族之间没有统计学上显著的关系。
粪便微生物多样性和组成与 IBS 女性的日常肠外症状、粪便稠度和 QOL 有关。
