肠道纤维化的小鼠模型。
Mouse Models of Intestinal Fibrosis.
机构信息
Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Department of Gastroenterology, Hepatology and Nutrition, Digestive Diseases and Surgery Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
出版信息
Methods Mol Biol. 2021;2299:385-403. doi: 10.1007/978-1-0716-1382-5_26.
Abstract
Mouse models are essential for investigation of underlying disease mechanisms that drive intestinal fibrosis, as well as assessment of potential therapeutic approaches to either prevent or resolve fibrosis. Here we describe several common mouse models of intestinal inflammation and fibrosis, including chemically driven colitis models, a bacterially triggered colitis model, and spontaneous intestinal inflammation in genetically susceptible mouse strains. Detailed protocols are provided for dextran sodium sulfate (DSS) colitis, 2,4,6-trinitro-benzene sulfonic acid (TNBS) colitis, adherent-invasive Escherichia coli (AIEC)-triggered colitis, the interleukin-10 knockout (IL-10KO) mouse model of spontaneous colitis, and the SAMP/YitFc model of spontaneous ileocolitis.
摘要
小鼠模型对于研究导致肠道纤维化的潜在疾病机制以及评估预防或解决纤维化的潜在治疗方法至关重要。在这里，我们描述了几种常见的肠道炎症和纤维化的小鼠模型，包括化学诱导的结肠炎模型、细菌触发的结肠炎模型和遗传易感小鼠品系中的自发性肠道炎症。我们提供了详细的葡聚糖硫酸钠（DSS）结肠炎、2,4,6-三硝基苯磺酸（TNBS）结肠炎、黏附侵袭性大肠杆菌（AIEC）触发的结肠炎、白细胞介素-10 敲除（IL-10KO）小鼠自发性结肠炎模型以及自发性回肠炎 SAMP/YitFc 模型的实验方案。
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