De Marchis Francesca, Bellucci Michele
Research Division of Perugia, Institute of Biosciences and Bioresources (IBBR), CNR, Perugia, Italy.
Methods Mol Biol. 2021;2317:283-290. doi: 10.1007/978-1-0716-1472-3_16.
Chloroplast biotechnology has assumed great importance in the past 20 years and, thanks to the numerous advantages as compared to conventional transgenic technologies, has been applied in an increasing number of plant species but still very much limited. Hence, it is of outmost importance to extend the range of species in which plastid transformation can be applied. Sugar beet (Beta vulgaris L.) is an important industrial crop of the temperate zone in which chloroplast DNA is not transmitted trough pollen. Transformation of the sugar beet genome is performed in several research laboratories, conversely sugar beet plastome genetic transformation is far away from being considered a routine technique. We describe here a method to obtain transplastomic sugar beet plants trough biolistic transformation. The availability of sugar beet transplastomic plants should avoid the risk of gene flow between these cultivated genetic modified sugar beet plants and the wild-type plants or relative wild species.
在过去20年里，叶绿体生物技术已变得极为重要。由于与传统转基因技术相比具有众多优势，它已被应用于越来越多的植物物种，但应用范围仍然非常有限。因此，扩大可应用质体转化的物种范围至关重要。甜菜（Beta vulgaris L.）是温带地区一种重要的经济作物，其叶绿体DNA不会通过花粉传递。几个研究实验室都在进行甜菜基因组的转化，相反，甜菜质体基因组的遗传转化远未被视为一种常规技术。我们在此描述一种通过生物枪转化获得转基因甜菜植株的方法。转基因甜菜植株的可得性应可避免这些转基因栽培甜菜植株与野生型植株或相关野生种之间基因流动的风险。
