Department of Neuroscience and Center for Neurovirology, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA.
Department of Biology, College of Science & Technology, Temple University, Philadelphia, PA, USA.
Methods Mol Biol. 2021;2311:1-8. doi: 10.1007/978-1-0716-1437-2_1.
In this introductory chapter, we provide a general overview of neuronal cell culture. This is a rapidly evolving area of research and we provide an outline and contextual framework for the different chapters of this book. These chapters have all been contributed by scientists actively working in the field who are currently using state-of-the-art techniques to advance our understanding of the molecular and cellular biology of the central nervous system. Each chapter provides detailed descriptions and experimental protocols for a variety of techniques ranging in scope from basic neuronal cell line culturing to advanced and specialized methods.
在本章导言中,我们将对神经元细胞培养进行概述。该领域的研究进展迅速,我们为本书的不同章节提供了一个概述和背景框架。这些章节的内容均由该领域的活跃科研人员撰写,他们目前正利用最先进的技术来增进我们对中枢神经系统的分子和细胞生物学的理解。各章节详细介绍了各种技术的实验方案,从基本的神经元细胞系培养到先进的专门方法,涵盖范围广泛。