International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, India.
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, India.
Microb Pathog. 2021 Aug;157:104961. doi: 10.1016/j.micpath.2021.104961. Epub 2021 May 24.
Three strains of Streptomyces griseus (CAI-24, CAI-121 and CAI-127) and one strain each of Streptomyces africanus (KAI-32) and Streptomyces coelicolor (KAI-90) were reported by us as biocontrol agents against Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceri (FOC), and as plant growth-promoters (PGP) in chickpea. In the present study, the combined effect of these Streptomyces strains as a consortium were assessed for their biocontrol potential against Fusarium wilt and PGP in chickpea. Based on their compatibility, biocontrol ability and PGP performance, two consortia were assembled, consortium-1 having all the five strains of Streptomyces sp. and consortium-2 having the two promising strains (CAI-127 and KAI-32). Under greenhouse conditions, consortium-1 and consortium-2 were found to reduce the Fusarium wilt disease incidence by 55% and 74%, while under field conditions, these were by 86% and 96% in year-1 and by 54% and 69% in year-2, respectively, when compared to the positive control (only FOC treated). Shoot samples treated with consortia + FOC contained significantly enhanced antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase and phenylalanine ammonia-lyase, when compared to the positive control (only FOC treated) or the negative control samples (neither FOC nor consortia treated). When the consortia were evaluated for their PGP traits under field conditions in two chickpea cultivars, JG11 and ICCV2, and in two consecutive years, nodule number was found to enhance up to 25%, nodule weight up to 49%, leaf area up to 37%, leaf weight up to 43%, root weight up to 23%, shoot weight up to 35%, seed weight up to 30%, seed number up to 29%, total dry matter up to 22% and grain yield up to 22% over the un-inoculated control plants. This study had demonstrated that the selected consortium of Streptomyces spp. has a greater potential for biological control of Fusarium wilt disease and PGP in chickpea.
我们曾报道过三株灰色链霉菌（CAI-24、CAI-121 和 CAI-127）和一株非洲链霉菌（KAI-32）以及一株天蓝链霉菌（KAI-90）可以作为生物防治剂来防治由尖孢镰刀菌古巴专化型（FOC）引起的枯萎病，并能促进鹰嘴豆的生长。在本研究中，评估了这些链霉菌菌株作为一个联合体的联合防治潜力，以对抗枯萎病和促进鹰嘴豆的生长。基于它们的相容性、生物防治能力和 PGP 性能，我们组装了两个联合体，联合体 1 由所有 5 株链霉菌组成，联合体 2 由两种有前途的菌株（CAI-127 和 KAI-32）组成。在温室条件下，联合体 1 和联合体 2 使枯萎病发病率降低了 55%和 74%，而在田间条件下，与阳性对照（仅用 FOC 处理）相比，第一年分别降低了 86%和 96%，第二年分别降低了 54%和 69%。与阳性对照（仅用 FOC 处理）或阴性对照样品（既不用 FOC 也不用联合体处理）相比，用联合体+FOC 处理的芽样品中含有明显增强的抗氧化酶，如超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、愈创木酚过氧化物酶、谷胱甘肽还原酶和苯丙氨酸解氨酶。当联合体在两个鹰嘴豆品种（JG11 和 ICCV2）和两个连续年份的田间条件下评估其 PGP 特性时，发现根瘤数增加了 25%，根瘤重量增加了 49%，叶面积增加了 37%，叶重增加了 43%，根重增加了 23%，茎重增加了 35%，种子重量增加了 30%，种子数量增加了 29%，总干物质增加了 22%，籽粒产量增加了 22%，而未接种对照植物的产量则没有增加。本研究表明，所选的链霉菌联合体具有更大的潜力，可以在鹰嘴豆中进行生物防治枯萎病和促进生长。
