Gopalakrishnan Subramaniam, Srinivas Vadlamudi, Sree Vidya Meesala, Rathore Abhishek
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502 324 Andhra Pradesh India.
Springerplus. 2013 Oct 29;2:574. doi: 10.1186/2193-1801-2-574. eCollection 2013.
Five strains of Streptomyces (CAI-24, CAI-121, CAI-127, KAI-32 and KAI-90) were earlier reported by us as biological control agents against Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri (FOC). In the present study, the Streptomyces were characterized for enzymatic activities, physiological traits and further evaluated in greenhouse and field for their plant growth promotion (PGP) of sorghum and rice. All the Streptomyces produced lipase, β-1-3-glucanase and chitinase (except CAI-121 and CAI-127), grew in NaCl concentrations of up to 6%, at pH values between 5 and 13 and temperatures between 20 and 40°C and were highly sensitive to Thiram, Benlate, Captan, Benomyl and Radonil at field application level. When the Streptomyces were evaluated in the greenhouse on sorghum all the isolates significantly enhanced all the agronomic traits over the control. In the field, on rice, the Streptomyces significantly enhanced stover yield (up to 25%; except CAI-24), grain yield (up to 10%), total dry matter (up to 18%; except CAI-24) and root length, volume and dry weight (up to 15%, 36% and 55%, respectively, except CAI-24) over the control. In the rhizosphere soil, the Streptomyces significantly enhanced microbial biomass carbon (except CAI-24), nitrogen, dehydrogenase (except CAI-24), total N, available P and organic carbon (up to 41%, 52%, 75%, 122%, 53% and 13%, respectively) over the control. This study demonstrates that the selected Streptomyces which were antagonistic to FOC also have PGP properties.
我们之前报道过五株链霉菌（CAI - 24、CAI - 121、CAI - 127、KAI - 32和KAI - 90）可作为生物防治剂，用于防治由尖孢镰刀菌鹰嘴豆专化型（FOC）引起的鹰嘴豆枯萎病。在本研究中，对这些链霉菌进行了酶活性、生理特性表征，并在温室和田间进一步评估了它们对高粱和水稻的促生长作用（PGP）。所有链霉菌都产生脂肪酶、β - 1 - 3 - 葡聚糖酶和几丁质酶（CAI - 121和CAI - 127除外），能在高达6%的NaCl浓度、pH值为5至13以及温度为20至40°C的条件下生长，并且在田间施用水平下对福美双、苯菌灵、克菌丹、苯菌灵和咯菌腈高度敏感。当在温室中对高粱进行评估时，所有分离株均显著提高了所有农艺性状，优于对照。在田间，对于水稻，链霉菌显著提高了秸秆产量（高达25%；CAI - 24除外）、籽粒产量（高达10%）、总干物质（高达18%；CAI - 24除外）以及根长、根体积和根干重（分别高达15%、36%和55%，CAI - 24除外），优于对照。在根际土壤中，链霉菌显著提高了微生物生物量碳（CAI - 24除外）、氮、脱氢酶（CAI - 24除外）、总氮、有效磷和有机碳（分别高达41%、52%、75%、122%、53%和13%），优于对照。本研究表明，所选的对FOC具有拮抗作用的链霉菌也具有促生长特性。
