Wanner A, Barker J A, Long W M, Mariassy A T, Chediak A D
Department of Medicine, University of Miami, Mount Sinai Medical Center, Florida 33101.
J Appl Physiol (1985). 1988 Jul;65(1):264-71. doi: 10.1152/jappl.1988.65.1.264.
The purpose of this study was to develop and validate a new in vivo technique for the measurement of tracheal mucosal blood flow (Qtr) and tissue water volume (VH2O) with an inert soluble gas. The technique was based on the notion that the uptake of dimethyl ether (VDME) from an isolated tracheal segment is governed by VH2O (transient state) and Qtr (steady state). In lightly anesthetized sheep, an endotracheal tube with two cuffs placed 14.5-16.5 cm apart was placed to create a chamber into which dimethyl ether was introduced and from which VDMME into the mucosa was determined with a sensitive pneumotachograph. Mean Qtr was 1.20 ml/min (range 0.87-1.73), and mean VH2O was 1.67 ml (range 1.27-2.26). Qtr correlated with cardiac output but not with body weight or tracheal mucosal surface area determined by He dilution. VH2O did not show a correlation with any of these parameters. The response to selected pharmacological agents suggested that the measurements of Qtr and VH2O are independent of each other and from changes in tracheal diameter. Mean Qtr was 80% of mean tracheal mucosal blood flow measured with radiolabeled microspheres. We concluded that the inert soluble gas method is capable of measuring in vivo the perfusion and a water compartment of the intact tracheal mucosa.
本研究的目的是开发并验证一种新的体内技术，用于使用惰性可溶性气体测量气管黏膜血流（Qtr）和组织水含量（VH2O）。该技术基于这样的概念，即从离体气管段摄取二甲醚（VDME）受VH2O（瞬态）和Qtr（稳态）的控制。在轻度麻醉的绵羊中，放置一根带有两个相距14.5 - 16.5 cm袖带的气管插管，以形成一个腔室，向其中引入二甲醚，并使用灵敏的呼吸流速计测定进入黏膜的VDMME。平均Qtr为1.20 ml/分钟（范围0.87 - 1.73），平均VH2O为1.67 ml（范围1.27 - 2.26）。Qtr与心输出量相关，但与体重或通过氦稀释法测定的气管黏膜表面积无关。VH2O与这些参数中的任何一个均无相关性。对选定药理剂的反应表明，Qtr和VH2O的测量相互独立，且与气管直径的变化无关。平均Qtr为用放射性标记微球测量的平均气管黏膜血流的80%。我们得出结论，惰性可溶性气体法能够在体内测量完整气管黏膜的灌注和一个水室。
