Jeyanthi Venkadapathi, Velusamy Palaniyandi, Kumar Govindarajan Venkat, Kiruba Kannan
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, 603 203, India.
Department of Biotechnology, SRM Arts and Science College, Kattankulathur, Chengalpattu District, 603203, Tamil Nadu, India.
Heliyon. 2021 May 12;7(5):e07021. doi: 10.1016/j.heliyon.2021.e07021. eCollection 2021 May.
Nosocomial pathogens cause various health problems in human and many novel drugs are under investigation to combat the pathogens. The present study explains the naturally derived hydroquinone possible mode of action against MTCC 741 and MTCC 740. Time kill studies, cell viability assays, membrane potential assays, and potassium release assays were carried out to study the mode of action. Time kill studies revealed the rapid death of bacterial pathogens exposed to 4X MIC (Minimum inhibitory concentration) of the hydroquinone. Cell viability assay results showed that nearly half of the cell destruction of test pathogens occurred within one hour. Transmission electron microscopic (TEM) observations revealed the disruption of the cell membrane, which caused severe ultrastructural changes in both test pathogens. Hydroquinone dissipated the membrane potential of test pathogens, as confirmed by the depolarization of membrane potential, increases in permeability and leakage of intracellular potassium ions. At the concentration of 2X MIC hydroquinone in 5 min, about 91.41% and 84.85% potassium ions were released from MTCC 741 and MTCC 740, respectively. This is the first report on the mode of action of naturally derived hydroquinone against clinical pathogens.
医院病原体在人类中引发各种健康问题，许多新型药物正在研发以对抗这些病原体。本研究阐述了天然来源的对苯二酚对MTCC 741和MTCC 740可能的作用模式。进行了时间杀菌研究、细胞活力测定、膜电位测定和钾离子释放测定以研究其作用模式。时间杀菌研究显示，暴露于4倍最小抑菌浓度（MIC）对苯二酚的细菌病原体迅速死亡。细胞活力测定结果表明，测试病原体近一半的细胞破坏在一小时内发生。透射电子显微镜（TEM）观察显示细胞膜遭到破坏，这在两种测试病原体中均引起了严重的超微结构变化。膜电位去极化、通透性增加和细胞内钾离子泄漏证实，对苯二酚使测试病原体的膜电位消散。在5分钟内，2倍MIC浓度的对苯二酚作用下，MTCC 741和MTCC 740分别有大约91.41%和84.85%的钾离子释放。这是关于天然来源对苯二酚对临床病原体作用模式的首次报道。
