Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China.
Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
Plant Biotechnol J. 2021 Oct;19(10):2097-2112. doi: 10.1111/pbi.13640. Epub 2021 Jun 11.
Verticillium wilt is caused by the soil-borne vascular pathogen Verticillium dahliae, and affects a wide range of economically important crops, including upland cotton (Gossypium hirsutum). Previous studies showed that expression levels of BIN2 were significantly down-regulated during infestation with V. dahliae. However, the underlying molecular mechanism of BIN2 in plant regulation against V. dahliae remains enigmatic. Here, we characterized a protein kinase GhBIN2 from Gossypium hirsutum, and identified GhBIN2 as a negative regulator of resistance to V. dahliae. The Verticillium wilt resistance of Arabidopsis and cotton were significantly enhanced when BIN2 was knocked down. Constitutive expression of BIN2 attenuated plant resistance to V. dahliae. We found that BIN2 regulated plant endogenous JA content and influenced the expression of JA-responsive marker genes. Further analysis revealed that BIN2 interacted with and phosphorylated JAZ family proteins, key repressors of the JA signalling pathway in both Arabidopsis and cotton. Spectrometric analysis and site-directed mutagenesis showed that BIN2 phosphorylated AtJAZ1 at T196, resulting in the degradation of JAZ proteins. Collectively, these results show that BIN2 interacts with JAZ proteins and plays a negative role in plant resistance to V. dahliae. Thus, BIN2 may be a potential target gene for genetic engineering against Verticillium wilt in crops.
黄萎病是由土壤传播的维管束病原体大丽轮枝菌引起的，它影响着包括陆地棉（Gossypium hirsutum）在内的广泛的经济重要作物。先前的研究表明，在受到大丽轮枝菌侵染时，BIN2 的表达水平显著下调。然而，BIN2 在植物对大丽轮枝菌的调节中的潜在分子机制仍然是一个谜。在这里，我们从陆地棉中鉴定出一个蛋白激酶 GhBIN2，并确定 GhBIN2 是对大丽轮枝菌抗性的负调控因子。当 BIN2 被敲低时，拟南芥和棉花的黄萎病抗性显著增强。BIN2 的组成型表达减弱了植物对大丽轮枝菌的抗性。我们发现 BIN2 调节植物内源 JA 含量，并影响 JA 响应标记基因的表达。进一步的分析表明，BIN2 与 JAZ 家族蛋白相互作用并磷酸化它们，JAZ 家族蛋白是拟南芥和棉花中 JA 信号通路的关键抑制子。光谱分析和定点突变表明，BIN2 在 T196 处磷酸化 AtJAZ1，导致 JAZ 蛋白降解。总之，这些结果表明 BIN2 与 JAZ 蛋白相互作用，在植物对大丽轮枝菌的抗性中发挥负作用。因此，BIN2 可能是作物抗黄萎病遗传工程的一个潜在靶标基因。
