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海岛棉中 GbPP2C80 通过与 GbWAKL14 互作负调控 MPK3 和 ROS 信号通路对枯萎病和黄萎病的抗性

GbPP2C80 Interacts with GbWAKL14 to Negatively Co-Regulate Resistance to Fusarium and Verticillium wilt via MPK3 and ROS Signaling in Sea Island Cotton.

机构信息

Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, 830091, China.

出版信息

Adv Sci (Weinh). 2024 Aug;11(30):e2309785. doi: 10.1002/advs.202309785. Epub 2024 Jun 18.

DOI:10.1002/advs.202309785
PMID:38889299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11321686/
Abstract

Fusarium wilt (FW) is widespread in global cotton production, but the mechanism underlying FW resistance in superior-fiber-quality Sea Island cotton is unclear. This study reveals that FW resistance has been the target of genetic improvement of Sea Island cotton in China since the 2010s. The key nonsynonymous single nucleotide polymorphism (SNP, T/C) of gene Gbar_D03G001670 encoding protein phosphatase 2C 80 (PP2C80) results in an amino acid shift (L/S), which is significantly associated with FW resistance of Sea Island cotton. Silencing GbPP2C80 increases FW resistance in Sea Island cotton, whereas overexpressing GbPP2C80 reduces FW resistance in Arabidopsis. GbPP2C80 and GbWAKL14 exist synergistically in Sea Island cotton accessions with haplotype forms "susceptible-susceptible" (TA) and "resistant-resistant" (CC), and interact with each other. CRISPR/Cas9-mediated knockout of GbWAKL14 enhances FW and Verticillium wilt (VW) resistance in upland cotton and overexpression of GbWAKL14 and GbPP2C80 weakens FW and VW resistance in Arabidopsis. GbPP2C80 and GbWAKL14 respond to FW and VW by modulating reactive oxygen species (ROS) content via affecting MPK3 expression. In summary, two tandem genes on chromosome D03, GbPP2C80, and GbWAKL14, functions as cooperative negative regulators in cotton wilt disease defense, providing novel genetic resources and molecular markers for the development of resistant cotton cultivars.

摘要

枯萎病（FW）广泛存在于全球棉花生产中，但优质海岛棉中 FW 抗性的机制尚不清楚。本研究表明，自 2010 年代以来，FW 抗性一直是中国海岛棉遗传改良的目标。基因 Gbar_D03G001670 编码蛋白磷酸酶 2C80（PP2C80）的关键非同义单核苷酸多态性（SNP，T/C）导致氨基酸置换（L/S），这与海岛棉的 FW 抗性显著相关。沉默 GbPP2C80 增加了海岛棉的 FW 抗性，而过表达 GbPP2C80 降低了拟南芥的 FW 抗性。GbPP2C80 和 GbWAKL14 在具有单倍型形式“感病-感病”（TA）和“抗病-抗病”（CC）的海岛棉品种中协同存在，并相互作用。CRISPR/Cas9 介导的 GbWAKL14 敲除增强了陆地棉对 FW 和黄萎病（VW）的抗性，而过表达 GbWAKL14 和 GbPP2C80 则削弱了拟南芥对 FW 和 VW 的抗性。GbPP2C80 和 GbWAKL14 通过影响 MPK3 表达来调节活性氧（ROS）含量来响应 FW 和 VW。综上所述，位于染色体 D03 上的两个串联基因 GbPP2C80 和 GbWAKL14 作为棉花枯萎病防御的协同负调控因子，为培育抗病棉花品种提供了新的遗传资源和分子标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/a807bb3dac3b/ADVS-11-2309785-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/a753b9456fde/ADVS-11-2309785-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/155ee1a6afa1/ADVS-11-2309785-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/1d6291dbdc3d/ADVS-11-2309785-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/8f67ba084e8c/ADVS-11-2309785-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/a807bb3dac3b/ADVS-11-2309785-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/2de18e7ed81b/ADVS-11-2309785-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/729ecbb6473d/ADVS-11-2309785-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/585df7de098a/ADVS-11-2309785-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/a753b9456fde/ADVS-11-2309785-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/155ee1a6afa1/ADVS-11-2309785-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/1d6291dbdc3d/ADVS-11-2309785-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/8f67ba084e8c/ADVS-11-2309785-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e84/11321686/a807bb3dac3b/ADVS-11-2309785-g003.jpg

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