Department of materials Engineering, Faculty of Materials and Industries Engineering, Noshirvani University of Technology, Babol, Iran.
Department of materials Engineering, Faculty of Materials and Industries Engineering, Noshirvani University of Technology, Babol 47148-71167, Iran.
Biomed Mater. 2021 Jun 14;16(4). doi: 10.1088/1748-605X/ac0590.
In the present study, β-tricalcium phosphate (β-TCP) scaffolds with various amounts of bredigite (Bre) were fabricated by the space holder method. The effect of bredigite content on the structure, mechanical properties,bioactivity, and cell viability was investigated. The structural assessment of the composite scaffolds presented interconnected pores with diameter of 300-500 μm with around 78%-82% porosity. The results indicated that the compressive strength of the scaffolds with 20% bredigite (1.91 MPa) was improved in comparison with scaffolds with 10% bredigite (0.52 MPa), due to the reduction of the average pore and grain sizes. Also, the results showed that the bioactivity and biodegradability of β-TCP/20Bre were better than that of β-TCP/10Bre. Besides, in this study, the release kinetics of ciprofloxacin (CPFX) loaded β-TCP/Bre composites as well as the ability of scaffolds to function as a sustained release drug carrier was investigated. Drug release pattern of β-TCP/bredigite-5CPFX scaffolds exhibited the rapid burst release of 43% for 3 h along with sustained release (82%) for 32 h which is favorable for bone infection treatment. Antibacterial tests revealed that the antibacterial properties of β-TCP/bredigite scaffolds are strongly related to the CPFX concentration, wherein the scaffold containing 5% CPFX showed the most significant zone of inhibition (33 ± 0.5 mm) against. The higher specific surface areas of nanostructure β-TCP/bredigite scaffolds containing CPFX lead to an initial rapid release followed by constant drug delivery. MTT assay showed that the cell viability of β-TCP/bredigite scaffold loading with up to 1%-3% CPFX (95 ± 2%), is greater than for scaffolds containing 5% CPFX (84 ± 2%). In Overall, it may suggested that β-TCP/bredigite containing 1%-3% CPFX possesses great cell viability and antibacterial activity and be employed as bactericidal biomaterials and bone infection treatment.
在本研究中，通过空间占位剂法制备了具有不同含量硅灰石（Bre）的β-磷酸三钙（β-TCP）支架。研究了硅灰石含量对结构、力学性能、生物活性和细胞活力的影响。复合材料支架的结构评估表明，具有 78%-82%孔隙率的互连孔的直径为 300-500μm。结果表明，由于平均孔径和晶粒尺寸的减小，含 20%硅灰石（1.91MPa）的支架的抗压强度与含 10%硅灰石（0.52MPa）的支架相比有所提高。此外，结果表明，β-TCP/20Bre 的生物活性和生物降解性优于β-TCP/10Bre。此外，在这项研究中，研究了载环丙沙星（CPFX）的β-TCP/Bre 复合材料的释放动力学以及支架作为缓释药物载体的能力。β-TCP/硅灰石-5CPFX 支架的药物释放模式显示，在 3 小时内迅速释放 43%，持续释放（82%）32 小时，有利于骨感染的治疗。抗菌试验表明，β-TCP/硅灰石支架的抗菌性能与 CPFX 的浓度密切相关，其中含有 5%CPFX 的支架对抑菌环的抑制作用最为明显（33±0.5mm）。含有 CPFX 的纳米结构β-TCP/硅灰石支架具有更高的比表面积，导致初始快速释放，随后持续药物输送。MTT 试验表明，载有 1%-3%CPFX（95±2%）的β-TCP/硅灰石支架的细胞活力大于载有 5%CPFX（84±2%）的支架。总体而言，这可能表明含 1%-3%CPFX 的β-TCP/硅灰石具有良好的细胞活力和抗菌活性，可作为杀菌生物材料和骨感染治疗。
